NDLI: Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

Content Provider	PubMed Central
Author	Van, Griethuysen Arjanne Pouw, Miranda Leeuwen, Nan Van Heck, Max Willemse, Piet Anton, Buiting Jan, Kluytmans
Copyright Year	1999
Abstract	The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methicillin-susceptible isolates from cultures of blood from consecutive patients. Also, 267 methicillin-resistant isolates that comprised 248 different phage types were tested. Methicillin resistance was defined as the presence of the mecA gene. Of the 267 mecA gene-positive isolates, 263 were positive by the MRSA screen test (sensitivity, 98.5%), and all the mecA-gene negative strains were negative by the MRSA screen test (specificity, 100%). The oxacillin agar screen test detected methicillin resistance in 250 of the mecA gene-positive isolates (sensitivity, 93.6%). The sensitivity of the MRSA screen test was statistically significantly higher than the sensitivity of the oxacillin agar screen test (P < 0.05). The MRSA screen test is a highly sensitive and specific test for the detection of methicillin resistance. Also, it offers results within half an hour and is easy to perform, which makes this test a valuable tool in the ongoing battle against methicillin-resistant S. aureus.
Starting Page	2789
File Format	PDF
ISSN	1098660X
e-ISSN	1098660X
Journal	Journal of Clinical Microbiology
Issue Number	9
Volume Number	37
Language	English
Publisher	American Society for Microbiology
Publisher Date	1999-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)

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Rapid slide latex agglutination test for detection of methicillin resistance in staphylococcus aureus (1999).

Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus

Rapid detection of methicillin resistance in staphylococcus aureus isolates by the mrsa-screen latex agglutination test (1999).

Rapid Detection of Methicillin Resistance in Staphylococcus aureus Isolates by the MRSA-Screen Latex Agglutination Test

Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus

Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests.

Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-Screen latex agglutination (1999)

Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus.

Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus.

Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

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Rapid slide latex agglutination test for detection of methicillin resistance in staphylococcus aureus (1999).

Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus

Rapid detection of methicillin resistance in staphylococcus aureus isolates by the mrsa-screen latex agglutination test (1999).

Rapid Detection of Methicillin Resistance in Staphylococcus aureus Isolates by the MRSA-Screen Latex Agglutination Test

Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus

Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests.

Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-Screen latex agglutination (1999)

Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus.

Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus.

Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus