NDLI: A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.

Please wait, while we are loading the content...

Year: 2016, Volume: 35

Year: 2015, Volume: 34

Year: 2014, Volume: 33

Year: 2013, Volume: 33

Year: 2013, Volume: 32

Year: 2012, Volume: 32

Year: 2012, Volume: 31

Year: 2011, Volume: 30

Year: 2010, Volume: 29

Year: 2009, Volume: 28

Year: 2008, Volume: 27

Year: 2007, Volume: 26

Year: 2006, Volume: 26

Year: 2006, Volume: 25

Year: 2005, Volume: 24

Year: 2004, Volume: 24

Year: 2004, Volume: 23

Year: 2003, Volume: 22

Year: 2002, Volume: 21

Year: 2001, Volume: 20

Year: 2000, Volume: 19

Year: 1999, Volume: 18

Year: 1998, Volume: 17

Year: 1997, Volume: 16

Year: 1996, Volume: 15

Year: 1995, Volume: 14

Year: 1994, Volume: 13

Year: 1993, Volume: 12

Year: 1992, Volume: 11

Year: 1991, Volume: 10

Year: 1990, Volume: 9

Year: 1989, Volume: 8

Year: 1988, Volume: 7

Year: 1987, Volume: 6

Year: 1986, Volume: 5

Year: 1985, Volume: 4

Year: 1984, Volume: 3

Year: 1984, Volume: 3, Issue: 13

Year: 1984, Volume: 3, Issue: 12

Direct gene transfer to plants

Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens

Initiation of translation of the cauliflower mosaic virus genome from a polycistronic mRNA: evidence from deletion mutagenesis

A site-specific mutation within the active site of ribulose-1,5-bisphosphate carboxylase of Rhodospirillum rubrum

Structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization

Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat

Transient paralysis by heat shock of hormonal regulation of gene expression.

The nucleotide sequences recognized by the glucocorticoid receptor in the rabbit uteroglobin gene region are located far upstream from the initiation of transcription.

Termination of the ovalbumin gene transcription.

Human globin gene transcription in injected Xenopus oocytes: enhancement by sodium butyrate.

Structural features of yeast tRNA genes which affect transcription factor binding.

The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.

Multiple non-allelic genes encoding pancreatic alpha-amylase of mouse are expressed in a strain-specific fashion.

Characterization of two genes required for the position-effect control of yeast mating-type genes.

Cloning of the RNA2 gene of Saccharomyces cerevisiae.

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

On the role of the engrailed+ gene in the internal organs of Drosophila.

Lampbrush chromosome loop-specificity of transcript morphology in spermatocyte nuclei of Drosophila hydei

Phosphate incorporation into secretory protein of Chironomus salivary glands occurs during translation

Mechanism of UTP-modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription.

Regulation of a new bacteriophage T4 gene, 69, that spans an origin of DNA replication.

Note added in proof

The change of DNA structure by specific binding of the cAMP receptor protein from rotation diffusion and dichroism measurements.

Recombination efficiency is a quadratic function of the length of homology during plasmid transformation of Bacillus subtilis protoplasts and Escherichia coli competent cells.

Environmental temperature regulates transcription of a virulence pili operon in E. coli.

Neutralization of heat-labile toxin of E. coli by antibodies to synthetic peptides derived from the B subunit of cholera toxin.

Escherichia coli ribosomes translate in vivo with variable rate.

Note added in proof

Flagella and motility behaviour of square bacteria.

DNA sequencing with direct blotting electrophoresis.

Inhibition of proteolytic cleavage of the hemagglutinin of influenza virus by the calcium-specific ionophore A23187.

Covalently closed circles of human adenovirus DNA are infectious.

The first exon of region E1a genes of adenoviruses 5 and 12 encodes a separate functional protein domain.

A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.

Mechanism of activation of the mouse c-mos oncogene by the LTR of an intracisternal A-particle gene.

A novel combination of K-ras and myc amplification accompanied by point mutational activation of K-ras in a human lung cancer.

The myc proteins are not associated with chromatin in mitotic cells.

Note added in proof

c-myc and immunoglobulin kappa light chain constant genes are on the 8q+ chromosome of three Burkitt lymphoma lines with t(2;8) translocations.

PC13 embryonal carcinoma-derived growth factor.

Purification and properties of porcine platelet-derived growth factor.

Cellular distribution of three mammalian Ca2+-binding proteins related to Torpedo calelectrin.

Kinetics of internalisation and degradation of surface-bound interferon in human lymphoblastoid cells.

Monoclonal antibodies to endogenous galactose-specific tumor cell lectins.

Structure and polymorphism of the HLA class II SB light chain genes.

Tracts of high or low sequence divergence in the mouse major histocompatibility complex.

Molecular basis of an isogeneic anti-idiotypic response.

Activation of immunoglobulin mu gene expression involves stepwise demethylation.

VH genes encoding the immune response to beta-(1,6)-galactan: somatic mutation in IgM molecules.

Unrearranged immunoglobulin lambda variable region is transcribed in kappa-producing myelomas.

Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit

Year: 1984, Volume: 3, Issue: 11

Year: 1984, Volume: 3, Issue: 10

Year: 1984, Volume: 3, Issue: 9

Year: 1984, Volume: 3, Issue: 8

Year: 1984, Volume: 3, Issue: 7

Year: 1984, Volume: 3, Issue: 6

Year: 1984, Volume: 3, Issue: 5

Year: 1984, Volume: 3, Issue: 4

Year: 1984, Volume: 3, Issue: 3

Year: 1984, Volume: 3, Issue: 2

Year: 1984, Volume: 3, Issue: 1

Year: 1983, Volume: 2

Year: 1982, Volume: 1

A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.

Content Provider	PubMed Central
Author	Choder, M. Bratosin, S. Aloni, Y.
Abstract	A direct quantitative method was developed for detecting a small fraction of minichromosomes active in transcription and for analyzing their chromatin structure. For this, SV40 minichromosomes were incubated in vitro in the presence of [alpha-32P]UTP to allow elongation of the in vivo pre-initiated nascent RNA and thus labeling of the in vivo transcriptionally active molecules. Subsequently the minichromosomes were treated with Sarkosyl to remove most of the proteins bound to the viral DNA while leaving the 32P-labeled RNA-DNA-polymerase ternary complexes intact. Following agarose gel electrophoresis, the bulk SV40 DNA was identified by staining with ethidium bromide and the labeled ternary complexes by autoradiography, although they are not well separated in the gel. When the viral minichromosomes were cleaved with certain restriction enzymes or mildly with DNase I prior to the in vitro labeling and Sarkosyl treatment, analysis by gel electrophoresis showed that all transcriptionally active minichromosomes were cleaved, whereas a large fraction of the bulk minichromosomes was resistant to cleavage. In all molecules the cleavage occurred within the transcriptional regulatory region. In addition, analysis by electron microscopy revealed that SV40 minichromosomes having a nucleosome-free region (gap) are also more sensitive to the initial cleavage by both DNase I and Bg/I restriction enzyme than non-gapped molecules and that the initial DNase I cleavage occurs within the gap. Thus, at least in SV40, the nuclease-hypersensitive region is positively correlated with the existence of a gap.
Starting Page	2929
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	12
Volume Number	3
Language	English
Publisher Date	1984-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in