NDLI: Transcription of human c-myc in permeabilized nuclei is associated with formation of Z-DNA in three discrete regions of the gene.

Please wait, while we are loading the content...

Year: 2016, Volume: 35

Year: 2015, Volume: 34

Year: 2014, Volume: 33

Year: 2013, Volume: 33

Year: 2013, Volume: 32

Year: 2012, Volume: 32

Year: 2012, Volume: 31

Year: 2011, Volume: 30

Year: 2010, Volume: 29

Year: 2009, Volume: 28

Year: 2008, Volume: 27

Year: 2007, Volume: 26

Year: 2006, Volume: 26

Year: 2006, Volume: 25

Year: 2005, Volume: 24

Year: 2004, Volume: 24

Year: 2004, Volume: 23

Year: 2003, Volume: 22

Year: 2002, Volume: 21

Year: 2001, Volume: 20

Year: 2000, Volume: 19

Year: 1999, Volume: 18

Year: 1998, Volume: 17

Year: 1997, Volume: 16

Year: 1996, Volume: 15

Year: 1995, Volume: 14

Year: 1994, Volume: 13

Year: 1993, Volume: 12

Year: 1992, Volume: 11

Year: 1992, Volume: 11, Issue: 13

Year: 1992, Volume: 11, Issue: 12

Structural and functional studies on platelet-derived growth factor.

Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes.

Noted added in proof

Fibroblast growth factor receptor-4 shows novel features in genomic structure, ligand binding and signal transduction.

Structure of recombinant N-terminal globule of type VI collagen alpha 3 chain and its binding to heparin and hyaluronan.

The multidrug resistance and cystic fibrosis genes have complementary patterns of epithelial expression.

The atypical M2 segment of the beta subunit confers picrotoxinin resistance to inhibitory glycine receptor channels.

The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.

Cytokine effects of CD23 are mediated by an epitope distinct from the IgE binding site.

Development and function of T cells in mice with a disrupted CD2 gene.

What limits affinity maturation of antibodies in Xenopus--the rate of somatic mutation or the ability to select mutants?

Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.

Noted added in proof

Isolation and cloning of Omp alpha, a coiled-coil protein spanning the periplasmic space of the ancestral eubacterium Thermotoga maritima.

Reversible phosphorylation--dephosphorylation determines the localization of rab4 during the cell cycle.

The internalization signal in the cytoplasmic tail of lysosomal acid phosphatase consists of the hexapeptide PGYRHV.

Identification of two structurally related proteins involved in proteolytic processing of precursors targeted to the chloroplast.

Cytoplasmic delivery of ribozymes leads to efficient reduction in alpha-lactalbumin mRNA levels in C127I mouse cells.

Activation of HIV-1 pre-mRNA 3' processing in vitro requires both an upstream element and TAR.

Native mRNA editing complexes from Trypanosoma brucei mitochondria.

Expression of the E.coli 3-methyladenine DNA glycosylase I gene in mammalian cells reduces the toxic and mutagenic effects of methylating agents.

High plasticity of multispecific DNA methyltransferases in the region carrying DNA target recognizing enzyme modules.

Epimutation of repeated genes in Ascobolus immersus.

Frequent transpositions of Drosophila melanogaster HeT-A transposable elements to receding chromosome ends.

Cell lineage-specific expression of modulo, a dose-dependent modifier of variegation in Drosophila.

Mapping of the in vivo start site for leading strand DNA synthesis in plasmid R1.

HU, the major histone-like protein of E. coli, modulates the binding of IHF to oriC.

SRY, like HMG1, recognizes sharp angles in DNA.

Determination of the base recognition positions of zinc fingers from sequence analysis.

Laminin A chain: expression during Drosophila development and genomic sequence.

Neuronal specificity of the alpha 7 nicotinic acetylcholine receptor promoter develops during morphogenesis of the central nervous system.

Regulatory pathways governing modulation of fungal gene expression by a virulence-attenuating mycovirus.

p21ras mediates control of IL-2 gene promoter function in T cell activation.

GATA-1 but not SCL induces megakaryocytic differentiation in an early myeloid line.

Analysis of the human alpha globin upstream regulatory element (HS-40) in transgenic mice.

A regulatory element that mediates co-operation between a PEA3-AP-1 element and an AP-1 site is required for phorbol ester induction of urokinase enhancer activity in HepG2 hepatoma cells.

Sequence-specific initiator elements focus initiation of transcription to distinct sites in the yeast TRP4 promoter.

A novel downstream regulatory element of the mouse H-2Kb class I major histocompatibility gene.

The 55 kDa regulatory subunit of protein phosphatase 2A plays a role in the activation of the HPV16 long control region in human cells with a deletion in the short arm of chromosome 11.

Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1.

Functional antagonism between members of the myb family: B-myb inhibits v-myb-induced gene activation.

Spatial flexibility in ternary complexes between SRF and its accessory proteins.

Hormone-regulated v-rel estrogen receptor fusion protein: reversible induction of cell transformation and cellular gene expression.

Transcription of human c-myc in permeabilized nuclei is associated with formation of Z-DNA in three discrete regions of the gene.

A system to study transcription by yeast RNA polymerase I within the chromosomal context: functional analysis of the ribosomal DNA enhancer and the RBP1/REB1 binding sites.

Year: 1992, Volume: 11, Issue: 11

Year: 1992, Volume: 11, Issue: 10

Year: 1992, Volume: 11, Issue: 9

Year: 1992, Volume: 11, Issue: 8

Year: 1992, Volume: 11, Issue: 7

Year: 1992, Volume: 11, Issue: 6

Year: 1992, Volume: 11, Issue: 5

Year: 1992, Volume: 11, Issue: 4

Year: 1992, Volume: 11, Issue: 3

Year: 1992, Volume: 11, Issue: 2

Year: 1992, Volume: 11, Issue: 1

Year: 1991, Volume: 10

Year: 1990, Volume: 9

Year: 1989, Volume: 8

Year: 1988, Volume: 7

Year: 1987, Volume: 6

Year: 1986, Volume: 5

Year: 1985, Volume: 4

Year: 1984, Volume: 3

Year: 1983, Volume: 2

Year: 1982, Volume: 1

Transcription of human c-myc in permeabilized nuclei is associated with formation of Z-DNA in three discrete regions of the gene.

Content Provider	PubMed Central
Author	Wittig, B. Wölfl, S. Dorbic, T. Vahrson, W. Rich, A.
Abstract	When human U937 cells are placed in agarose microbeads and treated with a detergent, the cytoplasmic membrane is lysed and the nuclear membrane is permeabilized. However, the nuclei remain intact and maintain both replication and transcription. Biotin labeled monoclonal antibodies against Z-DNA have been diffused into this system and used to measure the amount of Z-DNA present in the nuclei. It has previously been shown that the amount of Z-DNA present decreases due to relaxation by topoisomerase I and increases as the level of transcription increases. Here we measure the formation of Z-DNA in the c-myc gene by crosslinking the antibodies to DNA using laser radiation at 266 nm for 10 ns. The crosslinked DNA is isolated by restriction digestion, separation of antibody labeled fractions through the biotin residue, and subsequent proteolysis to remove the crosslinked antibody. Three AluI restriction fragments of the c-myc gene are shown to form Z-DNA when the cell is transcribing c-myc. The Z-DNA forming segments are near the promoter regions of the gene. However, when U937 cells start to differentiate and transcription of the c-myc gene is down-regulated, the Z-DNA content goes to undetectable levels within 30-60 min.
Starting Page	4653
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	12
Volume Number	11
Language	English
Publisher Date	1992-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in