NDLI: Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody

Content Provider	PubMed Central
Author	Enari, Masato Flechsig, Eckhard Weissmann, Charles
Copyright Year	2001
Abstract	Exposure of susceptible neuroblastoma N2a cells to mouse scrapie prions leads to infection, as evidenced by the continued presence of the scrapie form of the prion protein (PrPSc) and infectivity after 300 or more cell doublings. We find that exposure to phosphatidylinositol-specific phospholipase C (PIPLC) or to the monoclonal anti-prion protein (PrP) antibody 6H4 not only prevents infection of susceptible N2a cells but also cures chronically scrapie-infected cultures, as judged by the long-term abrogation of PrPSc accumulation after cessation of treatment. A nonpassaged, stationary infected culture rapidly loses PrPSc when exposed to the antibody or PIPLC, indicating that the PrPSc level is determined by steady state equilibrium between formation and degradation, and that depletion of the cellular form of PrP can interrupt the propagation of PrPSc. These findings encourage the belief that passive immunization may provide a therapeutic approach to prion disease.
Related Links	http://dx.doi.org/10.1073/pnas.151242598
Ending Page	9299
Page Count	5
Starting Page	9295
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	16
Volume Number	98
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2001-07-31
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.

Direct Evidence of Generation and Accumulation of β-Sheet-rich Prion Protein in Scrapie-infected Neuroblastoma Cells with Human IgG1 Antibody Specific for β-Form Prion Protein*

Prion protein biosynthesis in scrapie-infected and uninfected neuroblastoma cells.

Direct Evidence of Generation and Accumulation of β-Sheet-rich Prion Protein in Scrapie-infected Neuroblastoma Cells with Human IgG1 Antibody Specific for β-Form Prion Protein

Antibody to DNA detects scrapie but not normal prion protein

Three scrapie prion isolates exhibit different accumulation patterns of the prion protein scrapie isoform.

Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep

Scrapie-infected murine neuroblastoma cells produce protease-resistant prion proteins.

Membrane Toxicity of Abnormal Prion Protein in Adrenal Chromaffin Cells of Scrapie Infected Sheep

Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody

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Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.

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