NDLI: Beringian Paleoecology Inferred from Permafrost-Preserved Fungal DNA†

Content Provider	PubMed Central
Author	Lydolph, Magnus C. Jacobsen, Jonas Arctander, Peter Gilbert, M. Thomas P. Gilichinsky, David A. Hansen, Anders J. Willerslev, Eske Lange, Lene
Copyright Year	2005
Abstract	The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used. A broad diversity of eukaryotic DNA sequences that were 510 bp long, including sequences of various fungi, plants, and invertebrates, could be obtained reproducibly from samples that were up to 300,000 to 400,000 years old. The sequences revealed that ancient fungal communities included a diversity of cold-adapted yeasts, dark-pigmented fungi, plant-parasitic fungi, and lichen mycobionts. DNA traces of tree-associated macrofungi in a modern tundra sample indicated that there was a shift in fungal diversity following the last ice age and supported recent results showing that there was a severe change in the plant composition in northeastern Siberia during this period. Interestingly, DNA sequences with high homology to sequences of coprophilic and keratinophilic fungi indicated that feces, hair, skin, and nails could have been sources of ancient megafauna DNA recently reported to be present in small amounts of Siberian permafrost sediments.
Related Links	http://dx.doi.org/10.1128/aem.71.2.1012-1017.2005
Ending Page	1017
Page Count	6
Starting Page	1012
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	2
Volume Number	71
Language	English
Publisher	American Society for Microbiology
Publisher Date	2005-02-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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