NDLI: Sorghum Genome Sequencing by Methylation Filtration

Content Provider	PubMed Central
Author	Bedell, Joseph A. Budiman, Muhammad A. Nunberg, Andrew Citek, Robert W. Robbins, Dan Jones, Joshua Flick, Elizabeth Rohlfing, Theresa Fries, Jason Bradford, Kourtney Mcmenamy, Jennifer Smith, Michael Holeman, Heather Roe, Bruce A. Wiley, Graham Korf, Ian F. Rabinowicz, Pablo D. Lakey, Nathan Mccombie, W. Richard Jeddeloh, Jeffrey A. Martienssen, Robert A.
Editor	Doebley, John
Copyright Year	2005
Abstract	Sorghum bicolor is a close relative of maize and is a staple crop in Africa and much of the developing world because of its superior tolerance of arid growth conditions. We have generated sequence from the hypomethylated portion of the sorghum genome by applying methylation filtration (MF) technology. The evidence suggests that 96% of the genes have been sequence tagged, with an average coverage of 65% across their length. Remarkably, this level of gene discovery was accomplished after generating a raw coverage of less than 300 megabases of the 735-megabase genome. MF preferentially captures exons and introns, promoters, microRNAs, and simple sequence repeats, and minimizes interspersed repeats, thus providing a robust view of the functional parts of the genome. The sorghum MF sequence set is beneficial to research on sorghum and is also a powerful resource for comparative genomics among the grasses and across the entire plant kingdom. Thousands of hypothetical gene predictions in rice and Arabidopsis are supported by the sorghum dataset, and genomic similarities highlight evolutionarily conserved regions that will lead to a better understanding of rice and Arabidopsis.
Related Links	http://dx.doi.org/10.1371/journal.pbio.0030013
Starting Page	13
File Format	PDF
ISSN	15457885
e-ISSN	15457885
Journal	PLoS Biology
Issue Number	1
Volume Number	3
Language	English
Publisher	Public Library of Science
Publisher Date	2005-01-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Agricultural and Biological Sciences(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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