NDLI: Expression of the E. coli fpg gene in mammalian cells reduces the mutagenicity of gamma-rays.

Please wait, while we are loading the content...

Year: 2016, Volume: 44

Year: 2015, Volume: 43

Year: 2014, Volume: 42

Year: 2013, Volume: 41

Year: 2012, Volume: 40

Year: 2011, Volume: 39

Year: 2010, Volume: 38

Year: 2009, Volume: 37

Year: 2008, Volume: 37

Year: 2008, Volume: 36

Year: 2007, Volume: 36

Year: 2007, Volume: 35

Year: 2006, Volume: 35

Year: 2006, Volume: 34

Year: 2005, Volume: 33

Year: 2004, Volume: 33

Year: 2004, Volume: 32

Year: 2003, Volume: 31

Year: 2002, Volume: 30

Year: 2001, Volume: 29

Year: 2000, Volume: 28

Year: 1999, Volume: 27

Year: 1998, Volume: 26

Year: 1997, Volume: 25

Year: 1996, Volume: 24

Year: 1995, Volume: 23

Year: 1994, Volume: 22

Year: 1994, Volume: 22, Issue: 25

Year: 1994, Volume: 22, Issue: 24

Year: 1994, Volume: 22, Issue: 23

Human and E.coli excinucleases are affected differently by the sequence context of acetylaminofluorene-guanine adduct.

Transcription factor AP-4 participates in activation of bovine leukemia virus long terminal repeat by p34 Tax.

The DNA sequence encompassing the transcription start site of a TATA-less promoter contains enough information to drive neuron-specific transcription.

A role for the human DNA repair enzyme HAP1 in cellular protection against DNA damaging agents and hypoxic stress.

Kinetic and equilibrium binding studies of the human papillomavirus type-16 transcription regulatory protein E2 interacting with core enhancer elements.

Two silencing sub-domains of v-erbA synergize with each other, but not with RXR.

Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro.

Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1.

Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor.

Role of a small RNA pol II subunit in TATA to transcription start site spacing.

Properties of damage-dependent DNA incision by nucleotide excision repair in human cell-free extracts.

Expression of the E. coli fpg gene in mammalian cells reduces the mutagenicity of gamma-rays.

An RNA-protein contact determined by 5-bromouridine substitution, photocrosslinking and sequencing.

Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs.

RNA polymerase II phosphorylation: uncoupling from GAL4-VP16 directed open complex formation and transcription in a reconstituted system.

Translation of 2'-modified mRNA in vitro and in vivo.

DNA binding specificity of the wheat bZIP protein EmBP-1.

Sequence-specific DNA double-strand breaks induced by triplex forming 125I labeled oligonucleotides.

The pseudodisaccharides: a novel class of group I intron splicing inhibitors.

Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain.

Mechanistic studies on depurination and apurinic site chain breakage in oligodeoxyribonucleotides.

Anti-human immunodeficiency virus type 1 activity of phosphorothioate analogs of oligodeoxynucleotides: penetration and localization of oligodeoxynucleotides in HIV-1-infected MOLT-4 cells.

Introduction of YACs into intact yeast cells by a procedure which shows low levels of recombinagenicity and co-transformation.

Transcriptional induction of the mouse metallothionein-I gene in hydrogen peroxide-treated Hepa cells involves a composite major late transcription factor/antioxidant response element and metal response promoter elements.

CD and DNA binding studies of a proline repeat-containing segment of the replication arrest protein Tus.

Histidylation by yeast HisRS of tRNA or tRNA-like structure relies on residues -1 and 73 but is dependent on the RNA context.

Replication fork barriers in the Xenopus rDNA.

Regulation of influenza virus RNA polymerase activity by cellular and viral factors.

Molecular sequestration stabilizes CAP-DNA complexes during polyacrylamide gel electrophoresis.

A multifunctional expression vector for an anti-HIV-1 ribozyme that produces a 5'- and 3'-trimmed trans-acting ribozyme, targeted against HIV-1 RNA, and cis-acting ribozymes that are designed to bind to and thereby sequester trans-activator proteins such as Tat and Rev.

Unusual properties of genomic DNA molecules spanning the euchromatic-heterochromatic junction of a Drosophila minichromosome.

Selective chemical autoligation on a double-stranded DNA template.

Analysis of the micronuclear B type surface protein gene in Paramecium tetraurelia.

Novel clustering of Sp1 transcription factor binding sites at the transcription initiation site of the human muscle phosphofructokinase P1 promoter.

Characterization of the promoter for the human 85 kDa cytosolic phospholipase A2 gene.

Identification and characterization of multiple A/T-rich cis-acting elements that control expression from Dictyostelium actin promoters: the Dictyostelium actin upstream activating sequence confers growth phase expression and has enhancer-like properties.

Stochastic context-free grammars for tRNA modeling.

Alu repeated DNAs are differentially methylated in primate germ cells.

Conformation, hydrogen bonding and aggregate formation of guanosine 5'-monophosphate and guanosine in dimethylsulfoxide.

Rapid isolation of DNA sequences flanking microsatellite repeats.

Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA

Are there two DNA methyltransferase gene families in plant cells? A new potential methyltransferase gene isolated from an Arabidopsis thaliana genomic library

Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.

The 16S ribosomal RNA mutation database (16SMDB)

Year: 1994, Volume: 22, Issue: 22

Year: 1994, Volume: 22, Issue: 21

Year: 1994, Volume: 22, Issue: 20

Year: 1994, Volume: 22, Issue: 19

Year: 1994, Volume: 22, Issue: 18

Year: 1994, Volume: 22, Issue: 17

Year: 1994, Volume: 22, Issue: 16

Year: 1994, Volume: 22, Issue: 15

Year: 1994, Volume: 22, Issue: 14

Year: 1994, Volume: 22, Issue: 13

Year: 1994, Volume: 22, Issue: 12

Year: 1994, Volume: 22, Issue: 11

Year: 1994, Volume: 22, Issue: 10

Year: 1994, Volume: 22, Issue: 9

Year: 1994, Volume: 22, Issue: 8

Year: 1994, Volume: 22, Issue: 7

Year: 1994, Volume: 22, Issue: 6

Year: 1994, Volume: 22, Issue: 5

Year: 1994, Volume: 22, Issue: 4

Year: 1994, Volume: 22, Issue: 3

Year: 1994, Volume: 22, Issue: 2

Year: 1994, Volume: 22, Issue: 1

Year: 1993, Volume: 21

Year: 1992, Volume: 20

Year: 1991, Volume: 19

Year: 1990, Volume: 18

Year: 1989, Volume: 17

Year: 1988, Volume: 16

Year: 1987, Volume: 15

Year: 1986, Volume: 14

Year: 1985, Volume: 13

Year: 1984, Volume: 12

Year: 1983, Volume: 11

Year: 1982, Volume: 10

Year: 1981, Volume: 9

Year: 1980, Volume: 8

Year: 1979, Volume: 7

Year: 1979, Volume: 6

Year: 1978, Volume: 5

Year: 1977, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1974, Volume: 1

Expression of the E. coli fpg gene in mammalian cells reduces the mutagenicity of gamma-rays.

Content Provider	PubMed Central
Author	Laval, F.
Abstract	The E. coli fpg gene encodes the formamido-pyrimidine-DNA-glycosylase (FPG protein) which specifically removes the formamido-pyrimidine and C8-oxoGuanine residues from gamma-irradiated DNA. The fpg gene was ligated in the psV2 vector and transfected into the Chinese hamster CHO and V-79 cells. The transfected cells expressed a formamido-pyrimidine-DNA-glycosylase activity 30 to 40-fold over the constitutive level. The resistance of CHO and V-79 cells to the lethal effect of gamma-rays was similar in control and transfected cells. Furthermore CHO cells expressing the fpg gene had the same resistance to the lethal effect of hydrogen peroxide as control cells. However, the sensitivity to the mutagenic effect of gamma-rays, measured as 6-thioguanine resistance, decreased both in CHO and V-79 transfected cells. Since the lethal effect of gamma-rays was not modified in cells overproducing the FPG protein, the results suggest that this protein protects the cells against the mutagenic lesions formed by ionizing radiations, and among them C8-oxoguanine.
Starting Page	4943
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	23
Volume Number	22
Language	English
Publisher Date	1994-11-25
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in