NDLI: Assessment of DNA damage and repair in specific genomic regions by quantitative immuno-coupled PCR.

Please wait, while we are loading the content...

Year: 2016, Volume: 44

Year: 2015, Volume: 43

Year: 2014, Volume: 42

Year: 2013, Volume: 41

Year: 2012, Volume: 40

Year: 2011, Volume: 39

Year: 2010, Volume: 38

Year: 2009, Volume: 37

Year: 2008, Volume: 37

Year: 2008, Volume: 36

Year: 2007, Volume: 36

Year: 2007, Volume: 35

Year: 2006, Volume: 35

Year: 2006, Volume: 34

Year: 2005, Volume: 33

Year: 2004, Volume: 33

Year: 2004, Volume: 32

Year: 2003, Volume: 31

Year: 2002, Volume: 30

Year: 2001, Volume: 29

Year: 2000, Volume: 28

Year: 1999, Volume: 27

Year: 1998, Volume: 26

Year: 1997, Volume: 25

Year: 1996, Volume: 24

Year: 1995, Volume: 23

Year: 1994, Volume: 22

Year: 1994, Volume: 22, Issue: 25

Year: 1994, Volume: 22, Issue: 24

Year: 1994, Volume: 22, Issue: 23

Year: 1994, Volume: 22, Issue: 22

Year: 1994, Volume: 22, Issue: 21

Year: 1994, Volume: 22, Issue: 20

Year: 1994, Volume: 22, Issue: 19

Year: 1994, Volume: 22, Issue: 18

Year: 1994, Volume: 22, Issue: 17

Year: 1994, Volume: 22, Issue: 16

Year: 1994, Volume: 22, Issue: 15

Year: 1994, Volume: 22, Issue: 14

Year: 1994, Volume: 22, Issue: 13

Year: 1994, Volume: 22, Issue: 12

Summary: the modified nucleosides of RNA.

SolI, a novel isoschizomer of BamHI isolated from Streptoverticillium olivoverticillatum.

Creating new DNA binding specificities in the yeast transcriptional activator GCN4 by combining selected amino acid substitutions.

Use of the 1-(2-fluorophenyl)-4-methoxypiperidin-4-yl (Fpmp) and related protecting groups in oligoribonucleotide synthesis: stability of internucleotide linkages to aqueous acid.

Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.

A master sequence related to a free left Alu monomer (FLAM) at the origin of the B1 family in rodent genomes.

Eukaryotic selenocysteine inserting tRNA species support selenoprotein synthesis in Escherichia coli.

MyoD1 promoter autoregulation is mediated by two proximal E-boxes.

Reduced beta 2-microglobulin mRNA levels in transgenic mice expressing a designed hammerhead ribozyme.

Hydration of DNA in aqueous solution: NMR evidence for a kinetic destabilization of the minor groove hydration of d-(TTAA)2 versus d-(AATT)2 segments.

Footprinting RNA-protein complexes following gel retardation assays: application to the R-17-procoat-RNA and tat--TAR interactions.

c-Myc binds to 5' flanking sequence motifs of the dihydrofolate reductase gene in cellular extracts: role in proliferation.

Base mutation analysis of topoisomerase II-idarubicin-DNA ternary complex formation. Evidence for enzyme subunit cooperativity in DNA cleavage.

Interactions between yeast TFIIIB components.

Banded krait minor-satellite (Bkm)-associated Y chromosome-specific repetitive DNA in mouse.

Formation of adriamycin--DNA adducts in vitro.

Identification and analysis of the pseudoknot-containing gag-pro ribosomal frameshift signal of simian retrovirus-1.

DNA damage by anti-cancer agents resolved at the nucleotide level of a single copy gene: evidence for a novel binding site for cisplatin in cells.

Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.

Circular RNA oligonucleotides. Synthesis, nucleic acid binding properties, and a comparison with circular DNAs.

GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element.

Localization of the intrinsically bent DNA region upstream of the E.coli rrnB P1 promoter.

Assessment of DNA damage and repair in specific genomic regions by quantitative immuno-coupled PCR.

HOVERGEN: a database of homologous vertebrate genes.

Enhancement of selectivity in recognition of nucleic acids via chemical autoligation.

The full-length transcript of the I factor, a LINE element of Drosophila melanogaster, is a potential bicistronic RNA messenger.

Ribozyme mediated degradation of beta-amyloid peptide precursor mRNA in COS-7 cells.

Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific, human globin gene expression.

In vivo excision and amplification of large segments of the Escherichia coli genome.

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

Synthesis of specific diastereomers of a DNA methylphosphonate heptamer, d(CpCpApApApCpA), and stability of base pairing with the normal DNA octamer d(TPGPTPTPTPGPGPC).

Identification of a vitamin D responsive element in the promoter of the rat cytochrome P450(24) gene.

Apo CIII gene transcription is regulated by a cytokine inducible NF-kappa B element.

Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments.

A new homogeneous assay system for specific nucleic acid sequences: poly-dA and poly-A detection.

A general and fast method for mapping mutations on the Escherichia coli chromosome.

Enrichment of DNA fragments for deleted sequences in human genome with nuclease S1 digestion and PCR amplification.

Removal of t-butyldimethylsilyl protection in RNA-synthesis. Triethylamine trihydrofluoride (TEA, 3HF) is a more reliable alternative to tetrabutylammonium fluoride (TBAF).

The use of a tRNA as a transcriptional reporter: the T7 late promoter is extremely efficient in Escherichia coli but its transcripts are poorly expressed.

Linkage structures strongly influence the binding cooperativity of DNA intercalators conjugated to triplex forming oligonucleotides

Year: 1994, Volume: 22, Issue: 11

Year: 1994, Volume: 22, Issue: 10

Year: 1994, Volume: 22, Issue: 9

Year: 1994, Volume: 22, Issue: 8

Year: 1994, Volume: 22, Issue: 7

Year: 1994, Volume: 22, Issue: 6

Year: 1994, Volume: 22, Issue: 5

Year: 1994, Volume: 22, Issue: 4

Year: 1994, Volume: 22, Issue: 3

Year: 1994, Volume: 22, Issue: 2

Year: 1994, Volume: 22, Issue: 1

Year: 1993, Volume: 21

Year: 1992, Volume: 20

Year: 1991, Volume: 19

Year: 1990, Volume: 18

Year: 1989, Volume: 17

Year: 1988, Volume: 16

Year: 1987, Volume: 15

Year: 1986, Volume: 14

Year: 1985, Volume: 13

Year: 1984, Volume: 12

Year: 1983, Volume: 11

Year: 1982, Volume: 10

Year: 1981, Volume: 9

Year: 1980, Volume: 8

Year: 1979, Volume: 7

Year: 1979, Volume: 6

Year: 1978, Volume: 5

Year: 1977, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1974, Volume: 1

Assessment of DNA damage and repair in specific genomic regions by quantitative immuno-coupled PCR.

Content Provider	PubMed Central
Author	Denissenko, M. F. Venkatachalam, S. Yamasaki, E. F. Wani, A. A.
Abstract	Fine analysis of DNA damage and repair at the subgenomic level has indicated a microheterogeneity of DNA repair in mammalian cells, including human. In addition to the well established Southern hybridization-based approach to investigate gene-specific DNA damage and repair, alternative methods utilizing the sensitivity of PCR have been evaluated. The latter technique has relied on decreased PCR amplification due to damage in template DNA. We have developed a novel quantitative assay combining the selective recovery of DNA damage containing genomic fragments with the PCR amplification. DNA isolated from 7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE) treated human skin fibroblasts was immunoprecipitated with polyclonal antibody BP-1. Recovered target sequences were amplified by PCR using primers encompassing a 149 bp target region around codon 12 of the H-ras proto-oncogene. Quantitative DNA damage specific response was observed with nanogram amounts of genomic DNA. This approach allowed analysis of the initial DNA damage at a level less than 1 anti-BPDE adduct per 6.4 kbp ras gene fragment. Repair proficient GM637 cells exposed to 2 microM anti-BPDE showed a faster removal of the adducts from the H-ras gene segment than from the genome overall. Gene-specific repair was not apparent in GM4429 xeroderma pigmentosum (complementation group A) cells. The established technique could be extended to the quantitative measurement of the repair of diverse DNA base lesions in any genomic region of known sequence.
Starting Page	2351
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	12
Volume Number	22
Language	English
Publisher Date	1994-06-25
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in