NDLI: Transcriptional Transitions during Dictyostelium Spore Germination†

Content Provider	PubMed Central
Author	Xu, Qikai Ibarra, Miroslava Mahadeo, Dana Shaw, Chad Huang, Eryong Adam, Kuspa Cotter, David Gad, Shaulsky
Copyright Year	2004
Abstract	Many protozoa form spores in response to adversity; therefore, spore germination is a key process in their life cycle. Dictyostelium discoideum sporulates in response to starvation following a developmental program. Germination is characterized by two visible changes, spore swelling and the emergence of amoeba from the spore capsule. Several studies have indicated that an additional process termed spore activation is also required, but the physiological changes that characterize the three phases are largely uncharacterized. We used microarrays to monitor global transcriptional transitions as a surrogate measure of the physiological changes that occur during germination. Using two independent methods to induce germination, we identified changes in mRNA levels that characterized the germination process rather than changes that resulted from the induction method. We found that germination is characterized by three transitions. The first transition occurs during activation, while the spores appear dormant, the largest transition occurs when swelling begins, and the third transition occurs when emergence begins. These findings indicate that activation and swelling are not passive occurrences, such as dilution of inhibitors or spore rehydration, but are active processes that are accompanied by dramatic events in mRNA degradation and de novo transcription. These findings confirm and extend earlier reports that genes such as celA are regulated during spore germination. We also found by mutation analysis that the unconventional myosin gene myoI, which is induced during early germination, plays roles in the maintenance of dormancy and in spore swelling. This finding suggests that some of the observed transcriptional changes are required for spore germination.
Related Links	http://dx.doi.org/10.1128/ec.3.5.1101-1110.2004
Ending Page	1110
Page Count	10
Starting Page	1101
File Format	PDF
ISSN	15359778
e-ISSN	15359786
Journal	Eukaryotic Cell
Issue Number	5
Volume Number	3
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Dictyostelium discoideum mRNAs developmentally regulated during spore germination have short half-lives.

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Characterization of cDNA clones specific for sequences developmentally regulated during Dictyostelium discoideum spore germination.

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