NDLI: IcmF and DotU Are Required for Optimal Effector Translocation and Trafficking of the Legionella pneumophila Vacuole

Content Provider	PubMed Central
Author	Vanrheenen, Susan M. Guillaume, Duménil Isberg, Ralph R.
Copyright Year	2004
Abstract	The gram-negative bacterium Legionella pneumophila causes a severe form of pneumonia called Legionnaires' disease, characterized by bacterial replication within alveolar macrophages. Prior to intracellular replication, the vacuole harboring the bacterium must first escape trafficking to the host lysosome, a process that is dependent on the Dot/Icm type IV secretion system. To identify genes required for intracellular growth, bacterial mutants were isolated that were delayed in escape from the macrophage but which retain a minimally functional Dot/Icm machinery. The mutations were found in eight distinct genes, including three genes known to be required for optimal intracellular growth. Two of these genes, icmF and dotU, are located at one end of a cluster of genes that encode the type IV secretion system, yet both icmF and dotU lack orthologs in other type IV translocons. DotU protein is degraded in the early postexponential phase in wild-type L. pneumophila and at all growth phases in an icmF mutant. IcmF contains an extracytoplasmic domain(s) based on accessibility to a membrane-impermeant amine-reactive reagent. In the absence of either gene, L. pneumophila targets inappropriately to LAMP-1-positive compartments during macrophage infection, is defective in the formation of replicative vacuoles, and is impaired in the translocation of the effector protein SidC. Therefore, although IcmF and DotU do not appear to be part of the core type IV secretion system, these proteins are necessary for an efficiently functioning secretion apparatus.
Related Links	http://dx.doi.org/10.1128/IAI.72.10.5972-5982.2004
Starting Page	5972
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	10
Volume Number	72
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Legionella pneumophila DotU and IcmF Are Required for Stability of the Dot/Icm Complex

Dot/Icm Effector Translocation by Legionella longbeachae Creates a Replicative Vacuole Similar to That of Legionella pneumophila despite Translocation of Distinct Effector Repertoires

Legionella pneumophila DotU and IcmF are required for stability of the Dot/Icm complex.

Characterization of the icmH and icmF Genes Required for Legionella pneumophila Intracellular Growth, Genes That Are Present in Many Bacteria Associated with Eukaryotic Cells

Legionella pneumophila Catalase-Peroxidases Are Required for Proper Trafficking and Growth in Primary Macrophages

The Legionella pneumophila icmGCDJBF Genes Are Required for Killing of Human Macrophages

Targeting of the Small GTPase Rab6A′ by the Legionella pneumophila Effector LidA

Lysine11-Linked Polyubiquitination of the AnkB F-Box Effector of Legionella pneumophila

Early Events in Phagosome Establishment Are Required for Intracellular Survival of Legionella pneumophila

IcmF and DotU Are Required for Optimal Effector Translocation and Trafficking of the Legionella pneumophila Vacuole

Similar Documents

Legionella pneumophila DotU and IcmF Are Required for Stability of the Dot/Icm Complex

Dot/Icm Effector Translocation by Legionella longbeachae Creates a Replicative Vacuole Similar to That of Legionella pneumophila despite Translocation of Distinct Effector Repertoires

Legionella pneumophila DotU and IcmF are required for stability of the Dot/Icm complex.

Characterization of the icmH and icmF Genes Required for Legionella pneumophila Intracellular Growth, Genes That Are Present in Many Bacteria Associated with Eukaryotic Cells

Legionella pneumophila Catalase-Peroxidases Are Required for Proper Trafficking and Growth in Primary Macrophages

The Legionella pneumophila icmGCDJBF Genes Are Required for Killing of Human Macrophages

Targeting of the Small GTPase Rab6A′ by the Legionella pneumophila Effector LidA

Lysine11-Linked Polyubiquitination of the AnkB F-Box Effector of Legionella pneumophila

Early Events in Phagosome Establishment Are Required for Intracellular Survival of Legionella pneumophila

IcmF and DotU Are Required for Optimal Effector Translocation and Trafficking of the Legionella pneumophila Vacuole