NDLI: Osmosensitivity Associated with Insertions in argP (iciA) or glnE in Glutamate Synthase-Deficient Mutants of Escherichia coli

Content Provider	PubMed Central
Author	Nandineni, Madhusudan R. Laishram, Rakesh S. Gowrishankar, J.
Copyright Year	2004
Abstract	An ampicillin enrichment strategy following transposon insertion mutagenesis was employed to obtain NaCl-sensitive mutants of a gltBD (glutamate synthase [GOGAT]-deficient) strain of Escherichia coli. It was reasoned that the gltBD mutation would sensitize the parental strain even to small perturbations affecting osmotolerance. Insertions conferring an osmosensitive phenotype were identified in the proU, argP (formerly iciA), and glnE genes encoding a glycine betaine/proline transporter, a LysR-type transcriptional regulator, and the adenylyltransferase for glutamine synthetase, respectively. The gltBD+ derivatives of the strains were not osmosensitive. The argP mutation, but not the glnE mutation, was associated with reduced glutamate dehydrogenase activity and a concomitant NH4 + assimilation defect in the gltBD strain. Supplementation of the medium with lysine or a lysine-containing dipeptide phenocopied the argP null mutation for both osmosensitivity and NH4 + assimilation deficiency in a gltBD background, and a dominant gain-of-function mutation in argP was associated with suppression of these lysine inhibitory effects. Osmosensitivity in the gltBD strains, elicited either by lysine supplementation or by introduction of the argP or glnE mutations (but not proU mutations), was also correlated with a reduction in cytoplasmic glutamate pools in cultures grown at elevated osmolarity. We propose that an inability to accumulate intracellular glutamate at high osmolarity underlies the osmosensitive phenotype of both the argP gltBD and glnE gltBD mutants, the former because of a reduction in the capacity for NH4 + assimilation into glutamate and the latter because of increased channeling of glutamate into glutamine.
Related Links	http://dx.doi.org/10.1128/jb.186.19.6391-6399.2004
Ending Page	6399
Page Count	9
Starting Page	6391
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	186
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli ▿

Roles of SpoT and FNR in NH4+ assimilation and osmoregulation in GOGAT (glutamate synthase)-deficient mutants of Escherichia coli.

Peptidase-deficient mutants of Escherichia coli.

Derepressed levels of glutamate synthase and glutamine synthetase in Escherichia coli mutants altered in glutamyl-transfer ribonucleic acid synthetase.

Escherichia coli mutants deficient in deoxyuridine triphosphatase.

Escherichia coli mutants deficient in exonuclease VII.

Recombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes.

Hemin-deficient mutants of Escherichia coli K-12.

New types of Escherichia coli recombination-deficient mutants.

Osmosensitivity Associated with Insertions in argP (iciA) or glnE in Glutamate Synthase-Deficient Mutants of Escherichia coli

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Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli ▿

Roles of SpoT and FNR in NH4+ assimilation and osmoregulation in GOGAT (glutamate synthase)-deficient mutants of Escherichia coli.

Peptidase-deficient mutants of Escherichia coli.

Derepressed levels of glutamate synthase and glutamine synthetase in Escherichia coli mutants altered in glutamyl-transfer ribonucleic acid synthetase.

Escherichia coli mutants deficient in deoxyuridine triphosphatase.

Escherichia coli mutants deficient in exonuclease VII.

Recombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes.

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New types of Escherichia coli recombination-deficient mutants.

Osmosensitivity Associated with Insertions in argP (iciA) or glnE in Glutamate Synthase-Deficient Mutants of Escherichia coli