NDLI: A simple technique for generating probes for RNA in situ hybridization: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster.

Please wait, while we are loading the content...

Year: 2016, Volume: 113

Year: 2015, Volume: 112

Year: 2014, Volume: 111

Year: 2013, Volume: 110

Year: 2012, Volume: 109

Year: 2011, Volume: 109

Year: 2011, Volume: 108

Year: 2010, Volume: 107

Year: 2009, Volume: 106

Year: 2009, Volume: 94

Year: 2008, Volume: 105

Year: 2007, Volume: 104

Year: 2006, Volume: 103

Year: 2005, Volume: 103

Year: 2005, Volume: 102

Year: 2004, Volume: 101

Year: 2003, Volume: 100

Year: 2002, Volume: 99

Year: 2001, Volume: 98

Year: 2000, Volume: 97

Year: 1999, Volume: 96

Year: 1998, Volume: 95

Year: 1997, Volume: 94

Year: 1996, Volume: 93

Year: 1995, Volume: 92

Year: 1994, Volume: 91

Year: 1993, Volume: 90

Year: 1992, Volume: 89

Year: 1991, Volume: 88

Year: 1991, Volume: 88, Issue: 24

Year: 1991, Volume: 88, Issue: 23

Year: 1991, Volume: 88, Issue: 22

Year: 1991, Volume: 88, Issue: 21

Year: 1991, Volume: 88, Issue: 20

Year: 1991, Volume: 88, Issue: 19

Year: 1991, Volume: 88, Issue: 18

Year: 1991, Volume: 88, Issue: 17

Year: 1991, Volume: 88, Issue: 16

Year: 1991, Volume: 88, Issue: 15

Year: 1991, Volume: 88, Issue: 14

Year: 1991, Volume: 88, Issue: 13

Year: 1991, Volume: 88, Issue: 12

Year: 1991, Volume: 88, Issue: 11

Year: 1991, Volume: 88, Issue: 10

Year: 1991, Volume: 88, Issue: 9

Identification of LRF-1, a leucine-zipper protein that is rapidly and highly induced in regenerating liver.

Possible catalytic activity of DNA in the reaction between the antitumor drug cis-diamminedichloroplatinum(II) and the intercalator N-methyl-2,7-diazapyrenium.

Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.

Functional analysis of human cardiac gap junction channel mutants.

Analysis of the genes involved in the insulin transmembrane mitogenic signal in Chinese hamster ovary cells, CHO-K1, utilizing insulin-independent mutants.

Long-term inhibition of tumor growth by tumor necrosis factor in the absence of cachexia or T-cell immunity.

Isolation and characterization of two peptides with prolactin release-inhibiting activity from porcine hypothalami.

Trans-seasonal action of androgen in the control of spring courtship behavior in male red-sided garter snakes.

Subtraction inhibition combined with a spiking threshold accounts for cortical direction selectivity.

Neurotrophic protein S100 beta stimulates glial cell proliferation.

Characterization of DNA binding and retinoic acid binding properties of retinoic acid receptor.

PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues.

The contribution of DNA single-stranded order to the thermodynamics of duplex formation.

The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity.

A site-specific deletion in mitochondrial DNA of Podospora is under the control of nuclear genes.

Cytoplasmic protein binding to highly conserved sequences in the 3' untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells.

Interleukin 7 receptor ligation stimulates tyrosine phosphorylation, inositol phospholipid turnover, and clonal proliferation of human B-cell precursors.

Surface appearance and instability of empty H-2 class I molecules under physiological conditions.

Poliovirus can enter and infect mammalian cells by way of an intercellular adhesion molecule 1 pathway.

Protein kinase C mediates transient spinule-type neurite outgrowth in the retina during light adaptation.

The evolutionarily conserved Krüppel-associated box domain defines a subfamily of eukaryotic multifingered proteins.

Localization and synthesis of the hormone-binding regions of the human thyrotropin receptor.

Transient occurrence of extrachromosomal DNA of an Arabidopsis thaliana transposon-like element, Tat1.

Cell cycle-specific effects of lovastatin.

Reversal of age-related increase in brain protein oxidation, decrease in enzyme activity, and loss in temporal and spatial memory by chronic administration of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone.

Human monoamine oxidase A and B genes exhibit identical exon-intron organization.

Affinity capture of [Arg8]vasopressin-receptor complex using immobilized antisense peptide.

Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein.

Alpha B-crystallin is a small heat shock protein.

Vitamin D deficiency causes a selective reduction in deposition of transforming growth factor beta in rat bone: possible mechanism for impaired osteoinduction.

Calculation of protein conformation as an assembly of stable overlapping segments: application to bovine pancreatic trypsin inhibitor.

Retinoic acid stimulates neurite outgrowth in the amphibian spinal cord.

Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.

cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium.

Allelic diversity is generated by intraexon sequence exchange at the DRB1 locus of primates.

Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare).

Localization of a gene for progressive myoclonus epilepsy to chromosome 21q22.

Thymic targets for Abelson murine leukemia virus are early gamma/delta T lymphocytes.

Proenkephalin A in bone-derived cells.

Calcium/calmodulin-dependent protein kinase mediates a pathway for transcriptional regulation.

c-rel activates but v-rel suppresses transcription from kappa B sites.

Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity.

Sites of interaction of the CCA end of peptidyl-tRNA with 23S rRNA.

The ATF/CREB transcription factor-binding site in the polymerase beta promoter mediates the positive effect of N-methyl-N'-nitro-N-nitrosoguanidine on transcription.

Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.

Proliferation of smooth muscle cells after vascular injury is inhibited by an antibody against basic fibroblast growth factor.

Epidermis as the source of ecdysone in an argasid tick.

Niemann-Pick disease: a frequent missense mutation in the acid sphingomyelinase gene of Ashkenazi Jewish type A and B patients.

Cloning of the HLA class II region in yeast artificial chromosomes.

UGA is translated as cysteine in pheromone 3 of Euplotes octocarinatus.

Assembly and deacetylation of N-acetylglucosaminyl-plasmanylinositol in normal and affected paroxysmal nocturnal hemoglobinuria cells.

Anti-Müllerian hormone Bruxelles: a nonsense mutation associated with the persistent Müllerian duct syndrome.

Localization of D1 dopamine receptor mRNA in brain supports a role in cognitive, affective, and neuroendocrine aspects of dopaminergic neurotransmission.

The mouse tyrosinase promoter is sufficient for expression in melanocytes and in the pigmented epithelium of the retina.

The Drosophila homologue of vertebrate myogenic-determination genes encodes a transiently expressed nuclear protein marking primary myogenic cells.

DL-2-amino-4-phosphonobutyric acid does not eliminate "ON" responses in the visual system of goldfish.

Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation.

Functional analysis of a liver-specific enhancer of the hepatitis B virus.

Escherichia coli kgtP encodes an alpha-ketoglutarate transporter.

The different tissue transcription patterns of genes for HNF-1, C/EBP, HNF-3, and HNF-4, protein factors that govern liver-specific transcription.

Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin.

Constitutive and trophoblast-specific expression of a class of bovine interferon genes.

Transforming growth factor beta represses the actions of myogenin through a mechanism independent of DNA binding.

Introduction of cytochrome P450IA2 metabolic capability into cell lines genetically matched for DNA repair proficiency/deficiency.

Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6.

A subunit common to an IgG Fc receptor and the T-cell receptor mediates assembly through different interactions.

Differential signal transduction via T-cell receptor CD3 zeta 2, CD3 zeta-eta, and CD3 eta 2 isoforms.

Loss of heterozygosity on the short arm of chromosome 17 is associated with high proliferative capacity and DNA aneuploidy in primary human breast cancer.

Direct spread of reovirus from the intestinal lumen to the central nervous system through vagal autonomic nerve fibers.

Severe von Willebrand disease due to a defect at the level of von Willebrand factor mRNA expression: detection by exonic PCR-restriction fragment length polymorphism analysis.

Characterization of melanotropin-release-inhibiting factor (melanostatin) from frog brain: homology with human neuropeptide Y.

Replication terminator protein of Escherichia coli is a transcriptional repressor of its own synthesis.

Anticodon-dependent aminoacylation of a noncognate tRNA with isoleucine, valine, and phenylalanine in vivo.

Mutations in conserved yeast DNA primase domains impair DNA replication in vivo.

A post-transcriptional mechanism contributes to circadian cycling of a per-beta-galactosidase fusion protein.

Immunofluorescence localization of thyroid hormone receptor protein beta 1 and variant alpha 2 in selected tissues: cerebellar Purkinje cells as a model for beta 1 receptor-mediated developmental effects of thyroid hormone in brain.

Evidence that nerve growth factor dependence of sympathetic neurons for survival in vitro may be determined by levels of cytoplasmic free Ca2+.

Bursting electrical activity in pancreatic beta cells caused by Ca(2+)- and voltage-inactivated Ca2+ channels.

Nucleokinesis: distinct pattern of cell translocation in response to an autocrine motility factor-like substance or fibronectin.

Guanine nucleotide-binding regulatory proteins in retinal pigment epithelial cells.

Physiological role of epidermal growth factor on adipose tissue development in vivo.

Theoretical analysis of a physical mapping strategy using random single-copy landmarks.

Identification of genes governing filamentous growth and tumor induction by the plant pathogen Ustilago maydis.

A simple technique for generating probes for RNA in situ hybridization: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster.

Alternative splicing contributes to K+ channel diversity in the mammalian central nervous system.

Chlamydia trachomatis developmentally regulated protein is homologous to eukaryotic histone H1.

Role for the Epstein-Barr virus nuclear antigen 2 in viral promoter switching during initial stages of infection.

The nuclear protooncogenes c-jun and c-fos as regulators of DNA replication.

Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA.

Induction of c-fos mRNA and AP-1 DNA-binding activity by cAMP in cooperation with either the adenovirus 243- or the adenovirus 289-amino acid E1A protein.

Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I.

On the association of restriction fragment length polymorphisms across species boundaries.

Measurement of ligand-induced activation in single viable T cells using the lacZ reporter gene.

Thymic tumorigenesis induced by overexpression of p56lck.

IgM antigen receptor complex contains phosphoprotein products of B29 and mb-1 genes.

Human immunodeficiency virus envelope protein determines the site of virus release in polarized epithelial cells.

Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator.

Identification of intracellular receptor proteins for activated protein kinase C.

Construction and characterization of a single-chain catalytic antibody.

Exon amplification: a strategy to isolate mammalian genes based on RNA splicing.

Protein-protein interactions with the acidic COOH terminus of the single-stranded DNA-binding protein of the bacteriophage T4.

Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket.

Human brain prostaglandin D synthase has been evolutionarily differentiated from lipophilic-ligand carrier proteins.

Local production of tumor necrosis factor encoded by recombinant vaccinia virus is effective in controlling viral replication in vivo.

Primary structure of the merozoite surface antigen 1 of Plasmodium vivax reveals sequences conserved between different Plasmodium species.

Splicing of juvenile and adult tau mRNA variants is regulated by thyroid hormone.

Antibodies that neutralize human beta interferon biologic activity recognize a linear epitope: analysis by synthetic peptide mapping.

The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.

Acquisition and loss of a neuronal Ca2+/calmodulin-dependent protein kinase during neuronal differentiation.

A heat-labile protein of Chlamydia trachomatis binds to HeLa cells and inhibits the adherence of chlamydiae.

Transforming function of proto-ras genes depends on heterologous promoters and is enhanced by specific point mutations

Protein turnover as a component in the light/dark regulation of phosphoenolpyruvate carboxylase protein-serine kinase activity in C4 plants

Neuropathological changes in transgenic mice carrying copies of a transcriptionally activated Mos protooncogene

Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals.

Year: 1991, Volume: 88, Issue: 8

Year: 1991, Volume: 88, Issue: 7

Year: 1991, Volume: 88, Issue: 6

Year: 1991, Volume: 88, Issue: 5

Year: 1991, Volume: 88, Issue: 4

Year: 1991, Volume: 88, Issue: 3

Year: 1991, Volume: 88, Issue: 2

Year: 1991, Volume: 88, Issue: 1

Year: 1990, Volume: 87

Year: 1989, Volume: 86

Year: 1988, Volume: 85

Year: 1987, Volume: 84

Year: 1986, Volume: 83

Year: 1985, Volume: 82

Year: 1984, Volume: 81

Year: 1983, Volume: 80

Year: 1982, Volume: 79

Year: 1981, Volume: 78

Year: 1980, Volume: 77

Year: 1979, Volume: 76

Year: 1978, Volume: 75

Year: 1977, Volume: 74

Year: 1976, Volume: 73

Year: 1975, Volume: 72

Year: 1974, Volume: 71

Year: 1973, Volume: 70

Year: 1972, Volume: 69

Year: 1971, Volume: 68

Year: 1970, Volume: 67

Year: 1970, Volume: 66

Year: 1970, Volume: 65

Year: 1969, Volume: 64

Year: 1969, Volume: 63

Year: 1969, Volume: 62

Year: 1968, Volume: 61

Year: 1968, Volume: 60

Year: 1968, Volume: 59

Year: 1967, Volume: 58

Year: 1967, Volume: 57

Year: 1966, Volume: 56

Year: 1966, Volume: 55

Year: 1965, Volume: 54

Year: 1965, Volume: 53

Year: 1964, Volume: 52

Year: 1964, Volume: 51

Year: 1963, Volume: 50

Year: 1963, Volume: 49

Year: 1962, Volume: 48

Year: 1961, Volume: 47

Year: 1960, Volume: 46

Year: 1959, Volume: 45

Year: 1958, Volume: 44

Year: 1957, Volume: 43

Year: 1956, Volume: 42

Year: 1955, Volume: 41

Year: 1954, Volume: 40

Year: 1953, Volume: 39

Year: 1952, Volume: 38

Year: 1951, Volume: 37

Year: 1950, Volume: 36

Year: 1949, Volume: 35

Year: 1948, Volume: 34

Year: 1947, Volume: 33

Year: 1946, Volume: 32

Year: 1945, Volume: 31

Year: 1944, Volume: 30

Year: 1943, Volume: 29

Year: 1942, Volume: 28

Year: 1941, Volume: 27

Year: 1940, Volume: 26

Year: 1939, Volume: 25

Year: 1938, Volume: 24

Year: 1937, Volume: 23

Year: 1936, Volume: 22

Year: 1935, Volume: 21

Year: 1934, Volume: 20

Year: 1933, Volume: 19

Year: 1932, Volume: 18

Year: 1931, Volume: 17

Year: 1930, Volume: 16

Year: 1929, Volume: 15

Year: 1928, Volume: 14

Year: 1927, Volume: 13

Year: 1926, Volume: 12

Year: 1925, Volume: 11

Year: 1924, Volume: 10

Year: 1923, Volume: 9

Year: 1922, Volume: 8

Year: 1921, Volume: 7

Year: 1920, Volume: 6

Year: 1919, Volume: 5

Year: 1918, Volume: 4

Year: 1917, Volume: 3

Year: 1916, Volume: 2

Year: 1915, Volume: 1

A simple technique for generating probes for RNA in situ hybridization: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster.

Content Provider	PubMed Central
Author	Boehm, T. Gonzalez-sarmiento, R. Kennedy, M. Rabbitts, T. H.
Abstract	Two major problems have to be solved in studies of genes near breakpoints of chromosome abnormalities and in large-scale genomic mapping projects: (i) the identification of genes within the large amount on nontranscribed DNA and (ii) the determination of the tissues in which the identified genes are transcribed. In situ hybridization to mRNA is ideally suited to assess gene expression in all tissues but probe preparation presents major difficulties for adapting the technique for rapid screening. Here, we present a procedure to easily generate strand-specific DNA probes for in situ hybridization. In this method, a DNA fragment to be tested in uniformly labeled, denatured, and prehybridized to an excess of competitor single-stranded DNA corresponding to either positive or negative strands of the test fragment. No sequence information is needed. The prehybridized mixture is used directly for hybridization to whole embryo or tissue sections. We demonstrate the utility of this approach for any nonrepetitive fragment by using cDNA probes, intronless genomic probes, or genomic probes comprising transcribed and nontranscribed DNA. As an example, we show that mRNA for the recombination-activating genes (RAG) RAG-1 and RAG-2 is found in thymus of dE16 mouse embryos. Within the thymus, high levels of expression of RAG-1 and RAG-2 are detectable in the cortex but not in the medullary region. This supports the view that RAG-1 and RAG-2 expression is associated with cells known to actively rearrange antigen receptor loci.
Starting Page	3927
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	88
Language	English
Publisher Date	1991-05-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in