NDLI: Cleavage of deoxyoxanosine-containing oligodeoxyribonucleotides by bacterial endonuclease V

Content Provider	PubMed Central
Author	Hitchcock, Thomas M. Gao, Honghai Cao, Weiguo
Copyright Year	2004
Abstract	Oxanine (O) is a deamination product derived from guanine with the nitrogen at the N 1 position substituted by oxygen. Cytosine, thymine, adenine, guanine as well as oxanine itself can be incorporated by Klenow Fragment to pair with oxanine in a DNA template with similar efficiency, indicating that oxanine in DNA may cause various mutations. As a nucleotide, deoxyoxanosine may substitute for deoxyguanosine to complete a primer extension reaction. Endonuclease V, an enzyme known for its enzymatic activity on uridine-, inosine- and xanthosine-containing DNA, can cleave oxanosine-containing DNA at the second phosphodiester bond 3′ to the lesion. Mg2+ or Mn2+, and to a small extent Co2+ or Ni2+, support the oxanosine-containing DNA cleavage activity. All four oxanosine-containing base pairs (A/O, T/O, C/O and G/O) were cleaved with similar efficiency. The cleavage of double-stranded oxanosine-containing DNA was ∼6-fold less efficient than that of double-stranded inosine-containing DNA. Single-stranded oxanosine-containing DNA was cleaved with a lower efficiency as compared with double-stranded oxanosine-containing DNA. A metal ion enhances the binding of endonuclease V to double-stranded and single-stranded oxanosine-containing DNA 6- and 4-fold, respectively. Hypothetic models of oxanine-containing base pairs and deaminated base recognition mechanism are presented.
Related Links	http://dx.doi.org/10.1093/nar/gkh747
Ending Page	4080
Page Count	10
Starting Page	4071
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	13
Volume Number	32
Language	English
Publisher	Oxford University Press
Publisher Date	2004-07-16
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Cleavage of deoxyoxanosine-containing oligodeoxyribonucleotides by bacterial endonuclease V

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