NDLI: Dynamics of Colonization and Expression of Pathogenicity Related Genes in Fusarium oxysporum f.sp. ciceri during Chickpea Vascular Wilt Disease Progression

Content Provider	PubMed Central
Author	Upasani, Medha L. Gurjar, Gayatri S. Kadoo, Narendra Y. Gupta, Vidya S.
Editor	Sarrocco, Sabrina
Copyright Year	2016
Abstract	Fusarium wilt caused by Fusarium oxysporum f.sp. ciceri (Foc) is a constant threat to chickpea productivity in several parts of the world. Understanding the molecular basis of chickpea-Foc interaction is necessary to improve chickpea resistance to Foc and thereby the productivity of chickpea. We transformed Foc race 2 using green fluorescent protein (GFP) gene and used it to characterize pathogen progression and colonization in wilt-susceptible (JG62) and wilt-resistant (Digvijay) chickpea cultivars using confocal microscopy. We also employed quantitative PCR (qPCR) to estimate the pathogen load and progression across various tissues of both the chickpea cultivars during the course of the disease. Additionally, the expression of several candidate pathogen virulence genes was analyzed using quantitative reverse transcriptase PCR (qRT-PCR), which showed their characteristic expression in wilt-susceptible and resistant chickpea cultivars. Our results suggest that the pathogen colonizes the susceptible cultivar defeating its defense; however, albeit its entry in the resistant plant, further proliferation is severely restricted providing an evidence of efficient defense mechanism in the resistant chickpea cultivar.
Related Links	http://dx.doi.org/10.1371/journal.pone.0156490
Starting Page	156490
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	5
Volume Number	11
Language	English
Publisher	Public Library of Science
Publisher Date	2016-05-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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BIOCHEMICAL AND MOLECULAR ASPECTS OF WILT (Fusarium oxysporum f.sp. ciceri) IN CHICKPEA (Cicer arietinum L.).

MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f.sp. ciceri CAUSING CHICKPEA WILT THROUGH SSR MARKERS

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BIOCHEMICAL AND MOLECULAR ASPECTS OF Pseudomonas fluorescens INDUCED RESISTANCE AGAINTS WILT (fusarium oxysporum f.sp. ciceri) IN CHIKPEA (Cicer arietinum L.)

Studies on wilt (Fusarium oxysporum f.sp. ciceri) of chickpea (Cicer arientinum L.)

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Studies of management practices on Fusarium oxysporum f.sp. ciceri inciting wilt in chickpea

Dynamics of Colonization and Expression of Pathogenicity Related Genes in Fusarium oxysporum f.sp. ciceri during Chickpea Vascular Wilt Disease Progression

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