NDLI: In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer

Content Provider	PubMed Central
Author	Kim, Mi Hyun Kim, Min Hwan Kim, Kwang Seok Park, Myung-jin Jeong, Jae-hoon Park, Seung Woo Hoon, Ji Young Kim, Kwang Il Lee, Tae Sup Ryu, Phil Youl Kang, Joo Hyun Lee, Yong Jin
Copyright Year	2016
Abstract	There is increasing evidence that cancer contains cancer stem cells (CSCs) that are capable of regenerating a tumor following chemotherapy or radiotherapy. CD44 and CD133 are used to identify CSCs. This study investigated non-invasive in vivo monitoring of CD44-positive cancer stem-like cells in breast cancer by γ-irradiation using molecular image by fusing the firefly luciferase (fLuc) gene with the CD44 promoter. We generated a breast cancer cell line stably expressing fLuc gene by use of recombinant lentiviral vector controlled by CD44 promoter (MCF7-CL). Irradiated MCF7-CL spheres showed upregulated expression of CD44 and CD133, by immunofluorescence and flow cytometry. Also, gene expression levels of CSCs markers in irradiated spheres were clearly increased. CD44+ CSCs increased fLuc expression and tumor growth in vivo and in vitro. When MCF7-CL was treated with siCD44 and irradiated, CD44 expression was inhibited and cell survival ratio was decreased. MCF7-CL subsets were injected into the mice and irradiated by using a cobalt-60 source. Then, in vivo monitoring was performed to observe the bioluminescence imaging (BLI). When breast cancer was irradiated, relative BLI signal was increased, but tumor volume was decreased compared to non-irradiated tumor. These results indicate that increased CD44 expression, caused by general feature of CSCs by irradiation and sphere formation, can be monitored by using bioluminescence imaging. This system could be useful to evaluate CD44-expressed CSCs in breast cancer by BLI in vivo as well as in vitro for radiotherapy.
Related Links	http://dx.doi.org/10.3892/ijo.2016.3493
Ending Page	2286
Page Count	10
Starting Page	2277
File Format	PDF
ISSN	10196439
e-ISSN	17912423
Journal	International Journal of Oncology
Issue Number	6
Volume Number	48
Language	English
Publisher	D.A. Spandidos
Publisher Date	2016-04-01
Access Restriction	Open
Rights Holder	D.A. Spandidos
Subject Keyword	Cancer Research Oncology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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