NDLI: Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis

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Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis

Content Provider	PubMed Central
Author	Ferraro, Davide Champ, Jérôme Teste, Bruno Serra, Marco Malaquin, Laurent Viovy, Jean-louis De, Cremoux Patricia Stephanie, Descroix
Copyright Year	2016
Abstract	The development of precision medicine, together with the multiplication of targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing and the implementation of more complex decision trees, without cost increase or loss of robustness. We present a platform combining droplet microfluidics and magnetic tweezers, performing RNA purification, reverse transcription and amplification in a fully automated and programmable way, in droplets of 250nL directly sampled from a microtiter-plate. This platform decreases sample consumption about 100 fold as compared to current robotized platforms and it reduces human manipulations and contamination risk. The platform’s performance was first evaluated on cell lines, showing robust operation on RNA quantities corresponding to less than one cell, and then clinically validated with a cohort of 21 breast cancer samples, for the determination of their HER2 expression status, in a blind comparison with an established routine clinical analysis.
Related Links	http://dx.doi.org/10.1038/srep25540
Starting Page	25540
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	6
Language	English
Publisher	Nature Publishing Group
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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