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| Content Provider | PubMed Central |
|---|---|
| Author | Diaz, Ana Isabel Garcia Ben, Moyon Coan, Philip M. Alfazema, Neza Venda, Lara Woollard, Kevin Tim, Aitman |
| Copyright Year | 2016 |
| Abstract | The Wistar Kyoto (WKY) rat and the spontaneously hypertensive (SHR) rat inbred strains are well-established models for human crescentic glomerulonephritis (CRGN) and metabolic syndrome, respectively. Novel transgenic (Tg) strains add research opportunities and increase scientific value to well-established rat models. We have created two novel Tg strains using Sleeping Beauty transposon germline transgenesis, ubiquitously expressing green fluorescent protein (GFP) under the rat elongation factor 1 alpha (EF1a) promoter on the WKY and SHR genetic backgrounds. The Sleeping Beauty system functioned with high transgenesis efficiency; 75% of new rats born after embryo microinjections were transgene positive. By ligation-mediated PCR, we located the genome integration sites, confirming no exonic disruption and defining a single or low copy number of the transgenes in the new WKY-GFP and SHR-GFP Tg lines. We report GFP-bright expression in embryos, tissues and organs in both lines and show preliminary in vitro and in vivo imaging data that demonstrate the utility of the new GFP-expressing lines for adoptive transfer, transplantation and fate mapping studies of CRGN, metabolic syndrome and other traits for which these strains have been extensively studied over the past four decades. |
| Related Links | http://dx.doi.org/10.1242/dmm.024208 |
| Ending Page | 471 |
| Page Count | 9 |
| Starting Page | 463 |
| File Format | |
| ISSN | 17548403 |
| e-ISSN | 17548411 |
| Journal | Disease Models & Mechanisms |
| Issue Number | 4 |
| Volume Number | 9 |
| Language | English |
| Publisher | The Company of Biologists Ltd |
| Publisher Date | 2016-04-01 |
| Access Restriction | Open |
| Rights Holder | The Company of Biologists Ltd |
| Subject Keyword | Immunology and Microbiology (miscellaneous) Biochemistry, Genetics and Molecular Biology(all) Medicine (miscellaneous) Neuroscience (miscellaneous) Medicine(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology |
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