NDLI: Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

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Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Content Provider	PubMed Central
Author	Guo, Juanjuan Fu, Xiaoliang Liao, Huidan Hu, Zhenyu Long, Lingling Yan, Weitao Ding, Yanjun Zha, Lagabaiyila Guo, Yadong Yan, Jie Chang, Yunfeng Cai, Jifeng
Copyright Year	2016
Abstract	Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity and rectum, while Firmicutes and Bacteroidetes in the mouth and rectum, respectively, gradually decreased. In particular, the arrival and oviposition of sarcosaphagous insects had no obvious influence on bacterial taxa composition, but accelerated the loss of biomass. In contrast to the rectum, the microbial community structure in the buccal cavity of live rats differed considerably from that of rats immediately after death. Although this research indicates that bacterial communities can be used as a “microbial clock” for the estimation of post-mortem interval, further work is required to better understand this concept.
Related Links	http://dx.doi.org/10.1038/srep24197
Starting Page	24197
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	6
Language	English
Publisher	Nature Publishing Group
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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