NDLI: T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones

Content Provider	PubMed Central
Author	Theaker, Sarah M. Rius, Cristina Alexander, Greenshields-watson Lloyd, Angharad Andrew, Trimby Fuller, Anna Miles, John J. Cole, David K. Mark, Peakman Sewell, Andrew K. Dolton, Garry
Copyright Year	2016
Abstract	Isolation of peptide-specific T-cell clones is highly desirable for determining the role of T-cells in human disease, as well as for the development of therapies and diagnostics. However, generation of monoclonal T-cells with the required specificity is challenging and time-consuming. Here we describe a library-based strategy for the simple parallel detection and isolation of multiple peptide-specific human T-cell clones from CD8+ or CD4+ polyclonal T-cell populations. T-cells were first amplified by CD3/CD28 microbeads in a 96U-well library format, prior to screening for desired peptide recognition. T-cells from peptide-reactive wells were then subjected to cytokine-mediated enrichment followed by single-cell cloning, with the entire process from sample to validated clone taking as little as 6 weeks. Overall, T-cell libraries represent an efficient and relatively rapid tool for the generation of peptide-specific T-cell clones, with applications shown here in infectious disease (Epstein–Barr virus, influenza A, and Ebola virus), autoimmunity (type 1 diabetes) and cancer.
Related Links	http://dx.doi.org/10.1016/j.jim.2016.01.014
Ending Page	50
Page Count	8
Starting Page	43
File Format	PDF
ISSN	00221759
e-ISSN	18727905
Journal	Journal of Immunological Methods
Volume Number	430
Language	English
Publisher	Elsevier
Publisher Date	2016-03-01
Access Restriction	Open
Rights Holder	Elsevier
Subject Keyword	Immunology Immunology and Allergy Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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