NDLI: A non‐canonical function of Plk4 in centriolar satellite integrity and ciliogenesis through PCM1 phosphorylation

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A non‐canonical function of Plk4 in centriolar satellite integrity and ciliogenesis through PCM1 phosphorylation

Content Provider	PubMed Central
Author	Hori, Akiko Karin, Barnouin Snijders, Ambrosius P. Toda, Takashi
Abstract	Centrioles are the major constituents of the animal centrosome, in which Plk4 kinase serves as a master regulator of the duplication cycle. Many eukaryotes also contain numerous peripheral particles known as centriolar satellites. While centriolar satellites aid centriole assembly and primary cilium formation, it is unknown whether Plk4 plays any regulatory roles in centriolar satellite integrity. Here we show that Plk4 is a critical determinant of centriolar satellite organisation. Plk4 depletion leads to the dispersion of centriolar satellites and perturbed ciliogenesis. Plk4 interacts with the satellite component PCM1, and its kinase activity is required for phosphorylation of the conserved S372. The nonphosphorylatable PCM1 mutant recapitulates phenotypes of Plk4 depletion, while the phosphomimetic mutant partially rescues the dispersed centriolar satellite patterns and ciliogenesis in cells depleted of PCM1. We show that S372 phosphorylation occurs during the G1 phase of the cell cycle and is important for PCM1 dimerisation and interaction with other satellite components. Our findings reveal that Plk4 is required for centriolar satellite function, which may underlie the ciliogenesis defects caused by Plk4 dysfunction.
Related Links	http://dx.doi.org/10.15252/embr.201541432
Ending Page	337
Page Count	12
Starting Page	326
File Format	PDF
ISSN	1469221X
e-ISSN	14693178
Journal	EMBO Reports
Issue Number	3
Volume Number	17
Language	English
Publisher	John Wiley and Sons Inc.
Publisher Date	2016-03-01
Access Restriction	Open
Rights Holder	John Wiley and Sons Inc.
Subject Keyword	Genetics Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology

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