NDLI: Phosphorylation Regulates the Endocytic Function of the Yeast Dynamin-Related Protein Vps1

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Phosphorylation Regulates the Endocytic Function of the Yeast Dynamin-Related Protein Vps1

Content Provider	PubMed Central
Author	Rooij, Iwona I. Smaczynska-de Marklew, Christopher J. Allwood, Ellen G. Palmer, Sarah E. Booth, Wesley I. Mishra, Ritu Goldberg, Martin W. Ayscough, Kathryn R.
Copyright Year	2016
Abstract	The family of dynamin proteins is known to function in many eukaryotic membrane fusion and fission events. The yeast dynamin-related protein Vps1 functions at several stages of membrane trafficking, including Golgi apparatus to endosome and vacuole, peroxisomal fission, and endocytic scission. We have previously shown that in its endocytic role, Vps1 functions with the amphiphysin heterodimer Rvs161/Rvs167 to facilitate scission and release of vesicles. Phosphoproteome studies of Saccharomyces cerevisiae have identified a phosphorylation site in Vps1 at serine 599. In this study, we confirmed this phosphorylation event, and we reveal that, like Rvs167, Vps1 can be phosphorylated by the yeast cyclin-associated kinase Pho85 in vivo and in vitro. The importance of this posttranslational modification was revealed when mutagenesis of S599 to a phosphomimetic or nonphosphorylatable form caused defects in endocytosis but not in other functions associated with Vps1. Mutation to nonphosphorylatable valine inhibited the Rvs167 interaction, while both S599V and S599D caused defects in vesicle scission, as shown by both live-cell imaging and electron microscopy of endocytic invaginations. Our data support a model in which phosphorylation and dephosphorylation of Vps1 promote distinct interactions and highlight the importance of such regulatory events in facilitating sequential progression of the endocytic process.
Related Links	http://dx.doi.org/10.1128/mcb.00833-15
Ending Page	755
Page Count	14
Starting Page	742
File Format	PDF
ISSN	02707306
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	5
Volume Number	36
Language	English
Publisher	American Society for Microbiology
Publisher Date	2016-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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