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| Content Provider | PubMed Central |
|---|---|
| Author | Ruan, Wen-dong Wang, Pei Feng, Shiqing Xue, Yuan Zhang, Bin |
| Copyright Year | 2016 |
| Abstract | MicroRNAs (miRNAs) have a role in the development and progression of human malignancy. The expression of miR-497 is decreased in malignant tumors, which suggests a role for miR-497 as a tumor suppressor. Angiomotin is encoded by the AMOT gene, which is a target for miR-497. Angiomotin has a role in angiogenesis, cell proliferation, and invasion in human malignancies, including osteosarcoma. However, the role of miR-497 in human osteosarcoma is unknown. This preliminary study included human osteosarcoma tissues and normal tissues from 20 patients, the osteosarcoma cell lines, MG-63, SAOS-2, U-2 OS, and the human osteoblast cell line hFOB (OB3). Western blots for angiomotin and quantitative real-time polymerase chain reaction for the expression of miR-497 and AMOT were performed. Knockdown studies were performed using RNA interference and transfection studies used miR-497 mimics. Quantitative cell migration assays were performed, and cell apoptosis was studied by flow cytometry. Osteosarcoma cells and cell lines showed reduced expression of miR-497 and increased expression of angiomotin. Transfection of osteosarcoma cells with miR-497 mimics suppressed the expression of angiomotin. Results from a dual-luciferase reporter system supported AMOT as a direct target gene of miR-497. Knockdown of AMOT using RNA interference resulted in inhibition of osteosarcoma cell proliferation, migration, and invasion. These preliminary studies support a role for miR-497 as a suppressor of AMOT gene expression in human osteosarcoma cells, resulting in suppression of tumor cell proliferation and invasion. Further studies are recommended to investigate the role of miR-497 in osteosarcoma and other malignant mesenchymal tumors. |
| Related Links | http://dx.doi.org/10.2147/ott.s95204 |
| Ending Page | 313 |
| Starting Page | 303 |
| File Format | |
| ISSN | 11786930 |
| e-ISSN | 11786930 |
| Journal | OncoTargets and therapy |
| Volume Number | 9 |
| Language | English |
| Publisher | Dove Medical Press |
| Publisher Date | 2016-01-01 |
| Access Restriction | Open |
| Rights Holder | Dove Medical Press |
| Subject Keyword | Pharmacology (medical) Oncology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
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