NDLI: Monosodium Urate Crystal-Induced Chondrocyte Death via Autophagic Process

Content Provider	PubMed Central
Author	Hwang, Hyun Sook Yang, Chung Mi Park, Su Jin Kim, Hyun Ah
Editor	Mobasheri, Ali
Copyright Year	2015
Abstract	Monosodium urate (MSU) crystals, which are highly precipitated in the joint cartilage, increase the production of cartilage-degrading enzymes and pro-inflammatory mediators in cartilage, thereby leading to gouty inflammation and joint damage. In this study, we investigated the effect of MSU crystals on the viability of human articular chondrocytes and the mechanism of MSU crystal-induced chondrocyte death. MSU crystals significantly decreased the viability of primary chondrocytes in a time- and dose-dependent manner. DNA fragmentation was observed in a culture medium of MSU crystal-treated chondrocytes, but not in cell lysates. MSU crystals did not activate caspase-3, a marker of apoptosis, compared with actinomycin D and TNF-α-treated cells. MSU crystals did not directly affect the expression of endoplasmic reticulum (ER) stress markers at the mRNA and protein levels. However, MSU crystals significantly increased the LC3-II level in a time-dependent manner, indicating autophagy activation. Moreover, MSU crystal-induced autophagy and subsequent chondrocyte death were significantly inhibited by 3-methyladenine, a blocker of autophagosomes formation. MSU crystals activated autophagy via inhibition of phosporylation of the Akt/mTOR signaling pathway. These results demonstrate that MSU crystals may cause the death of chondrocytes through the activation of the autophagic process rather than apoptosis or ER stress.
Related Links	http://dx.doi.org/10.3390/ijms161226164
Ending Page	29277
Page Count	13
Starting Page	29265
File Format	PDF
ISSN	14220067
e-ISSN	14220067
Journal	International Journal of Molecular Sciences
Issue Number	12
Volume Number	16
Language	English
Publisher	MDPI
Publisher Date	2015-12-01
Access Restriction	Open
Rights Holder	MDPI
Subject Keyword	Physical and Theoretical Chemistry Inorganic Chemistry Organic Chemistry Spectroscopy Molecular Biology Catalysis Computer Science Applications Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Organic Chemistry Medicine Molecular Biology Physical and Theoretical Chemistry Catalysis Inorganic Chemistry Computer Science Applications

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Monosodium Urate Crystal-Induced Chondrocyte Death via Autophagic Process

Autophagic Cell Death and Cancer

UV-Induced Cell Death in Plants

Blocking Autophagic Flux Enhances Matrine-Induced Apoptosis in Human Hepatoma Cells

Tuning Fluidic Resistance via Liquid Crystal Microfluidics

Melatonin Prevents Chemical-Induced Haemopoietic Cell Death

Autophagic Cell Death and Apoptosis Jointly Mediate Cisatracurium Besylate-Induced Cell Injury

Chromatin Structure Following UV-Induced DNA Damage—Repair or Death?

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Monosodium Urate Crystal-Induced Chondrocyte Death via Autophagic Process

Similar Documents

Monosodium Urate Crystal-Induced Chondrocyte Death via Autophagic Process

Autophagic Cell Death and Cancer

UV-Induced Cell Death in Plants

Blocking Autophagic Flux Enhances Matrine-Induced Apoptosis in Human Hepatoma Cells

Tuning Fluidic Resistance via Liquid Crystal Microfluidics

Melatonin Prevents Chemical-Induced Haemopoietic Cell Death

Autophagic Cell Death and Apoptosis Jointly Mediate Cisatracurium Besylate-Induced Cell Injury

Chromatin Structure Following UV-Induced DNA Damage—Repair or Death?

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Monosodium Urate Crystal-Induced Chondrocyte Death via Autophagic Process