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| Content Provider | PubMed Central |
|---|---|
| Author | Wang, Ke-feng Yang, Hang Jiang, Wen-qi Li, Su Cai, Yu-chen |
| Copyright Year | 2015 |
| Abstract | There are numerous studies that demonstrate the anti-neoplastic activity of phosphatidylinositol 3-kinase (PI3K) inhibitors and the mechanisms of inducing autophagy in cancer cells. The new anticancer drug puquitinib mesylate (XC-302) is a molecular-targeted drug, which suppresses the activity of PI3K directly. However, it remains unclear whether XC-302 can develop an antitumor effect by inducing autophagy in naso-pharyngeal cancer cells. The MTT assay was used to study the anti-proliferative effects of XC-302. Subsequently, autophagy was determined by monodansylcadaverine (MDC) staining, punctate localization of green fluorescent protein (GFP)-light chain 3 (LC3), LC3 protein blotting and electron microscopy. The expression levels of beclin 1, p62, protein kinase B (AKT), phospho (p)-AKT, mechanistic target of rapamycin (mTOR) and p-mTOR in XC-302-induced autophagy were detected. Autophagy inhibition was assayed by 3-methyladenine (3-MA) or small interfering RNA (siRNA) silencing of beclin 1. XC-302 inhibited the viability of CNE-2 in a dose-dependent manner and the IC50 of 72 h was 5.2 µmol/l. After cells were exposed to XC-302 for 24 h, MDC-labeled autophagolysosomes were evident in CNE-2 cells by fluorescence microscope. Autophagosomes and autolysosomes were identified by transmission electron microscopy. Following transfection with GFP-LC3, XC-302 induced a significant accumulation of GFP-LC3, as monitored by a confocal microscope, which was reduced by 3-MA. XC-302 induced the formation of LC3-II, increased beclin 1 levels and decreased the expression of p62. Additionally, the expression levels of p-AKT and p-mTOR were reduced with the elevation of XC-302. Knockdown of beclin 1 with siRNA or co-treatment with 3-MA enhanced significantly the survival of CNE-2 and promoted the ability of clone formation. XC-302 also induced apoptosis in CNE-2, and when autophagy was inhibited by 3-MA, the apoptosis rate was decreased. The present data provides the evidence that XC-302 can induce autophagy in CNE-2, which promotes the program of cell death and inhibits the PI3K/AKT/mTOR signaling pathway. Furthermore, XC-302 also promoted apoptosis in CNE-2 cells, which could be reduced when autophagy was suppressed, meaning that autophagy may interact with apoptosis to induce cell death. |
| Related Links | http://dx.doi.org/10.3892/ijmm.2015.2378 |
| Ending Page | 1562 |
| Page Count | 7 |
| Starting Page | 1556 |
| File Format | |
| ISSN | 11073756 |
| e-ISSN | 1791244X |
| Journal | International Journal of Molecular Medicine |
| Issue Number | 6 |
| Volume Number | 36 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2015-10-01 |
| Access Restriction | Open |
| Rights Holder | D.A. Spandidos |
| Subject Keyword | Genetics Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
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