NDLI: Plasmid-encoded genes influence exosporium assembly and morphology in Bacillus megaterium QM B1551 spores

Content Provider	PubMed Central
Author	Julia, Manetsberger Hall, Elizabeth A. H. Christie, Graham
Editor	Ricca, Ezio
Copyright Year	2015
Abstract	Spores of Bacillus megaterium QM B1551 are encased in a morphologically distinctive exosporium. We demonstrate here that genes encoded on the indigenous pBM500 and pBM600 plasmids are required for exosporium assembly and or stability in spores of this strain. Bioinformatic analyses identified genes encoding orthologues of the B. cereus-family exosporium nap and basal layer proteins within the B. megaterium genome. Transcriptional analyses, supported by electron and fluorescent microscopy, indicate that the pole-localized nap, identified here for the first time in B. megaterium QM B1551 spores, is comprised of the BclA1 protein. The role of the BxpB protein, which forms the basal layer of the exosporium in B. cereus spores, is less clear since spores of a null mutant strain display an apparently normal morphology. Retention of the localized nap in bxpB null spores suggests that B. megaterium employs an alternative mechanism to that used by B. cereus spores in anchoring the nap to the spore surface.
Related Links	http://dx.doi.org/10.1093/femsle/fnv147
Starting Page	147
File Format	PDF
ISSN	15746968
e-ISSN	15746968
Journal	FEMS Microbiology Letters
Issue Number	18
Volume Number	362
Language	English
Publisher	Oxford University Press
Publisher Date	2015-09-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Microbiology

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