NDLI: The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa

Please wait, while we are loading the content...

The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa

Content Provider	PubMed Central
Author	Wang, Shiwei Liu, Xi Liu, Hongsheng Zhang, Li Guo, Yuan Shan, Yu Wozniak, Daniel J. Ma, Luyan Z.
Abstract	A hallmark of bacterial biofilms is a self-produced extracellular matrix of exopolysaccharide, extracellular DNA (eDNA) and proteins that hold bacterial cells together in the community. However, interactions among matrix components and how the interactions contribute to the formation of matrix remain unclear. Here, we show the physical interaction between exopolysaccharide Psl and eDNA, the two key biofilm matrix components of the opportunistic pathogen Pseudomonas aeruginosa. The interaction allows the two components to combine to form a web of eDNA–Psl fibres, which resembles a biofilm skeleton in the centre of pellicles to give bacteria structural support and capability against agents targeted on one matrix component. The web of eDNA–Psl fibres was also found in flow-cell biofilms at microcolonies initiation stage. The colocalization of eDNA or Psl fibres with bacterial cell membrane stain suggests that fibre-like eDNA is likely derived from the lysis of dead bacteria in biofilms. Psl can interact with DNA from diverse sources, suggesting that P. aeruginosa has the ability to use DNA of other organisms (such as human neutrophils and other bacterial species) to form its own communities, which might increase the survival of P. aeruginosa in multispecies biofilms or within a human host.
Related Links	http://dx.doi.org/10.1111/1758-2229.12252
Ending Page	340
Page Count	11
Starting Page	330
File Format	PDF
ISSN	17582229
e-ISSN	17582229
Journal	Environmental microbiology reports
Issue Number	2
Volume Number	7
Language	English
Publisher Date	2015-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The exopolysaccharide Psl-eDNA interaction enables the formation of a biofilm skeleton inPseudomonas aeruginosa

Identification of psl, a locus encoding a potential exopolysaccharide that is essential for pseudomonas aeruginosa pao1 biofilm formation (2004).

The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix

Pseudomonas aeruginosa Psl Exopolysaccharide Interacts with the Antimicrobial Peptide LG21

Identification of psl, a Locus Encoding a Potential Exopolysaccharide That Is Essential for Pseudomonas aeruginosa PAO1 Biofilm Formation

PAO 1 Biofilm Formation Pseudomonas aeruginosa Essential for Potential Exopolysaccharide That Is , a Locus Encoding a psl Identification of

The Biofilm Life-Cycle and Virulence of Pseudomonas aeruginosa are Dependent on a Filamentous Prophage

A biofilm-tropic Pseudomonas aeruginosa bacteriophage uses the exopolysaccharide Psl as receptor

Pseudomonas aeruginosa uses type III secretion system to kill biofilm-associated amoebae

The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa

Similar Documents

The exopolysaccharide Psl-eDNA interaction enables the formation of a biofilm skeleton inPseudomonas aeruginosa

Identification of psl, a locus encoding a potential exopolysaccharide that is essential for pseudomonas aeruginosa pao1 biofilm formation (2004).

The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix

Pseudomonas aeruginosa Psl Exopolysaccharide Interacts with the Antimicrobial Peptide LG21

Identification of psl, a Locus Encoding a Potential Exopolysaccharide That Is Essential for Pseudomonas aeruginosa PAO1 Biofilm Formation

PAO 1 Biofilm Formation Pseudomonas aeruginosa Essential for Potential Exopolysaccharide That Is , a Locus Encoding a psl Identification of

The Biofilm Life-Cycle and Virulence of Pseudomonas aeruginosa are Dependent on a Filamentous Prophage

A biofilm-tropic Pseudomonas aeruginosa bacteriophage uses the exopolysaccharide Psl as receptor

Pseudomonas aeruginosa uses type III secretion system to kill biofilm-associated amoebae

The exopolysaccharide Psl–eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa