Loading...
Please wait, while we are loading the content...
Development of an automated on-chip bead-based ELISA platform
| Content Provider | PubMed Central |
|---|---|
| Author | Campbell, Jennifer Pollock, Nira Sharon, Andre Sauer-budge, Alexis F. |
| Abstract | We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this “open source platform.” We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time. |
| Related Links | http://dx.doi.org/10.1039/c5ay00264h |
| Ending Page | 8477 |
| Page Count | 6 |
| Starting Page | 8472 |
| File Format | |
| ISSN | 17599660 |
| e-ISSN | 17599679 |
| Journal | Analytical methods : advancing methods and applications |
| Issue Number | 19 |
| Volume Number | 7 |
| Language | English |
| Publisher Date | 2015-01-01 |
| Access Restriction | Open |
| Subject Keyword | Engineering(all) Analytical Chemistry Chemical Engineering(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Engineering Chemical Engineering |
