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| Content Provider | PubMed Central |
|---|---|
| Author | Yang, Lei Mette, Lübeck Lübeck, Peter S. |
| Abstract | Aspergillus carbonarius has a potential as a cell factory for production of various organic acids. In this study, the organic acid profile of A. carbonarius was investigated under different cultivation conditions. Moreover, two heterologous genes, pepck and ppc, which encode phosphoenolpyruvate carboxykinase in Actinobacillus succinogenes and phosphoenolpyruvate carboxylase in Escherichia coli, were inserted individually and in combination in A. carbonarius to enhance the carbon flux toward the reductive TCA branch. Results of transcription analysis and measurement of enzyme activities of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase in the corresponding single and double transformants demonstrated that the two heterologous genes were successfully expressed in A. carbonarius. The production of citric acid increased in all the transformants in both glucose- and xylose-based media at pH higher than 3 but did not increase in the pH non-buffered cultivation compared with the wild type. |
| Related Links | http://dx.doi.org/10.1007/s10295-015-1688-4 |
| Ending Page | 1545 |
| Page Count | 13 |
| Starting Page | 1533 |
| File Format | |
| ISSN | 14765535 |
| e-ISSN | 14765535 |
| Journal | Journal of Industrial Microbiology & Biotechnology |
| Issue Number | 11 |
| Volume Number | 42 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-01-01 |
| Access Restriction | Open |
| Rights Holder | Springer Berlin Heidelberg |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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