NDLI: Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.

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Molecular recognition

Supramolecular effects and molecular discrimination by macrocyclic hosts embedded in synthetic bilayer membranes.

Molecular recognition analyzed by docking simulations: the aspartate receptor and isocitrate dehydrogenase from Escherichia coli.

Design of a metallo-bZIP protein that discriminates between CRE and AP1 target sites: selection against AP1.

A genetic approach to the generation of antibodies with enhanced catalytic activities.

Two light-transducing membrane proteins: bacteriorhodopsin and the mammalian rhodopsin.

Toward an estimation of binding constants in aqueous solution: studies of associations of vancomycin group antibiotics.

Energetics of cooperative binding of oligonucleotides with discrete dimerization domains to DNA by triple helix formation.

Anti-immune complex antibodies enhance affinity and specificity of primary antibodies.

Chemically bonded stationary phases that use synthetic hosts containing aromatic binding clefts: HPLC analysis of nitro-substituted polycyclic aromatic hydrocarbons.

Modeling the complexation of substituted benzenes by a cyclophane host in water.

Recognition and catalysis in nucleic acid chemistry.

Kinetics and mechanism in RNA cleavage.

Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli.

Actin is a binding protein for angiogenin.

Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity.

Identification of two distinct properties of class II major histocompatibility complex-associated peptides.

General Gittins index processes in discrete time.

Phosphoglucose isomerase: a ketol isomerase with aldol C2-epimerase activity.

Stimulation of Mn peroxidase activity: a possible role for oxalate in lignin biodegradation.

Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae.

Pertussis toxin-sensitive activation of p21ras by G protein-coupled receptor agonists in fibroblasts.

DNA homology requirements for mitotic gap repair in Drosophila.

T-cell receptor V beta expression in normal human skin.

A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens.

RNase H domain mutations affect the interaction between Moloney murine leukemia virus reverse transcriptase and its primer-template.

Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset.

A specific membrane binding protein for progesterone in rat brain: sex differences and induction by estrogen.

Futile cycles in Saccharomyces cerevisiae strains expressing the gluconeogenic enzymes during growth on glucose.

Stimulus-induced association of Ca(2+)-binding proteins with the plasma membrane detected in situ by photolabeling of intact chromaffin and PC12 cells.

Targeting RNA for degradation with a (2'-5')oligoadenylate-antisense chimera.

Catecholaminergic neurons result from intracerebral implantation of embryonal carcinoma cells.

Single-cell transplantation determines the time when Xenopus muscle precursor cells acquire a capacity for autonomous differentiation.

RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.

Transferrin in a cockroach: molecular cloning, characterization, and suppression by juvenile hormone.

Pathway of proton transfer in bacterial reaction centers: second-site mutation Asn-M44-->Asp restores electron and proton transfer in reaction centers from the photosynthetically deficient Asp-L213-->Asn mutant of Rhodobacter sphaeroides.

Displacement-clamp measurement of the forces exerted by gating springs in the hair bundle.

Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis.

ATP-modulated K+ channels sensitive to antidiabetic sulfonylureas are present in adenohypophysis and are involved in growth hormone release.

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Mechanism of superconductivity in K3C60.

Squalamine: an aminosterol antibiotic from the shark.

The HNK-1 reactive sulfoglucuronyl glycolipids are ligands for L-selectin and P-selectin but not E-selectin.

Evidence for functional interaction between elongation factor Tu and 16S ribosomal RNA.

5' control regions of the apolipoprotein(a) gene and members of the related plasminogen gene family.

Mitochondrial benzodiazepine receptor linked to inner membrane ion channels by nanomolar actions of ligands.

Three-dimensional profiles from residue-pair preferences: identification of sequences with beta/alpha-barrel fold.

Productivity, consumers, and the structure of a river food chain.

Assembly of avian mixed-species flocks in Amazonia.

Formation of large deletions by illegitimate recombination in the HPRT gene of primary human fibroblasts.

Sequence conversion during postreplicative adenovirus overlap recombination.

Correlation between Src family member regulation by the protein-tyrosine-phosphatase CD45 and transmembrane signaling through the T-cell receptor.

Telomere-like DNA polymorphisms associated with genetic predisposition to acute myeloid leukemia in irradiated CBA mice.

Combined use of an immunotoxin and cyclosporine to prevent both activated and quiescent peripheral blood T cells from producing type 1 human immunodeficiency virus.

Homozygous somatic Wt1 point mutations in sporadic unilateral Wilms tumor.

A beta-adrenergic receptor kinase-like enzyme is involved in olfactory signal termination.

Calcium binding proteins distinguish large and small cells of the ventral posterior and lateral geniculate nuclei of the prosimian galago and the tree shrew (Tupaia belangeri).

Reduction of exogenous vasopressin RNA poly(A) tail length increases its effectiveness in transiently correcting diabetes insipidus in the Brattleboro rat.

Interaction of the peroxisome-proliferator-activated receptor and retinoid X receptor.

Effects of genetic replacements of charged and H-bonding residues in the retinal pocket on Ca2+ binding to deionized bacteriorhodopsin.

Functional specificity of the homeodomain protein fushi tarazu: the role of DNA-binding specificity in vivo.

mRNA processing independent of RNase III and RNase E in the expression of the F1845 fimbrial adhesin of Escherichia coli.

Identification of a functional silencer element involved in neuron-specific expression of the synapsin I gene.

Guanine nucleotide binding protein involvement in early steps of phytochrome-regulated gene expression.

Identification of C-terminal amino acid residues of cauliflower mosaic virus open reading frame III protein responsible for its DNA binding activity.

Crystal structure of Escherichia coli L-asparaginase, an enzyme used in cancer therapy.

cDNA cloning of a human mRNA preferentially expressed in hematopoietic cells and with homology to a GDP-dissociation inhibitor for the rho GTP-binding proteins.

Using three-color chromosome painting to test chromosome aberration models.

T-DNA transfer to maize cells: histochemical investigation of beta-glucuronidase activity in maize tissues.

Identification of a nonprocessive telomerase activity from mouse cells.

Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.

Postproliferative transcription of the rat osteocalcin gene is reflected by vitamin D-responsive developmental modifications in protein-DNA interactions at basal and enhancer promoter elements.

Endogenous peptides bound to HLA-A3 possess a specific combination of anchor residues that permit identification of potential antigenic peptides.

Perivascular and intravenous administration of basic fibroblast growth factor: vascular and solid organ deposition.

Crystals of hemagglutinin-neuraminidase of parainfluenza virus contain triple-stranded helices.

Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family.

A Drosophila photoreceptor cell-specific protein, calphotin, binds calcium and contains a leucine zipper.

Human immunodeficiency virus coat protein gp120 inhibits the beta-adrenergic regulation of astroglial and microglial functions.

MEF2C, a MADS/MEF2-family transcription factor expressed in a laminar distribution in cerebral cortex.

Three unusual modifications, a D-arabinosyl, an N-methyl, and a carbamoyl group, are present on the Nod factors of Azorhizobium caulinodans strain ORS571.

5' sequences are important positive and negative determinants of the longevity of Chlamydomonas chloroplast gene transcripts.

The sulfolipid sulfoquinovosyldiacylglycerol is not required for photosynthetic electron transport in Rhodobacter sphaeroides but enhances growth under phosphate limitation.

Molecular basis of transabdominal--a sexually dimorphic mutant of the bithorax complex of Drosophila.

Electrostatic interactions modulate the RNA-binding and transactivation specificities of the human immunodeficiency virus and simian immunodeficiency virus Tat proteins.

In vitro selection of optimal DNA substrates for T4 RNA ligase.

The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB.

Regulation of peptide-calmodulin complexes by protein kinase C in vivo.

Normal development and growth of mice carrying a targeted disruption of the alpha 1 retinoic acid receptor gene.

Oscillatory flow in the cochlea visualized by a magnetic resonance imaging technique.

Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.

Stimulation of T cells by antigenic peptide complexed with isolated chains of major histocompatibility complex class II molecules.

Stimulation of human thyroid growth via the inhibitory guanine nucleotide binding (G) protein Gi: constitutive expression of the G-protein alpha subunit Gi alpha-1 in autonomous adenoma.

DNA repair and aging in basal cell carcinoma: a molecular epidemiology study.

Activation of c-myb is an early bone-marrow event in a murine model for acute promonocytic leukemia.

ARIA, a protein that stimulates acetylcholine receptor synthesis, also induces tyrosine phosphorylation of a 185-kDa muscle transmembrane protein.

Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase.

Micromotion of mammalian cells measured electrically

Diversity in the early Tertiary anthropoidean radiation in Africa

Characterization by yeast artificial chromosome cloning of the linked apolipoprotein(a) and plasminogen genes and identification of the apolipoprotein(a) 5′ flanking region

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Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.

Content Provider	PubMed Central
Author	Quadt, R. Kao, C. C. Browning, K. S. Hershberger, R. P. Ahlquist, P.
Abstract	The association of host proteins with viral RNA replication proteins has been reported for a number of (+)-strand RNA viruses. However, little is known about the identity or function of these host proteins in viral replication. In this paper we report the characterization of a host protein associated with the RNA-dependent RNA polymerase (RdRp) from brome mosaic virus (BMV)-infected barley. A host protein was specifically and proportionally enriched with BMV RdRp activity through several purification steps. This RdRp-associated host protein reacted with an antiserum prepared against wheat germ eukaryotic translation initiation factor 3 (eIF-3). The RdRp-associated host protein, the p41 subunit of wheat germ eIF-3, and an antigenically related protein from rabbit reticulocyte lysates were all found to bind with high affinity and specificity to BMV-encoded protein 2a, which is involved in viral RNA replication. Moreover, addition of wheat germ eIF-3 or the p41 subunit from wheat germ to BMV RdRp gave a specific and reproducible 3-fold stimulation of (-)-strand RNA synthesis in vivo. These results suggest that the barley analog of eIF-3 subunit p41, or a closely related protein, associates with BMV RdRp in vivo and is involved in BMV RNA replication. This observation and the established role of translation factors in bacteriophage Q beta RdRp suggest that association with translation factors may be a general feature of RNA replication by (+)-strand RNA viruses.
Starting Page	1498
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	90
Language	English
Publisher Date	1993-02-15
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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