NDLI: Effects of mutations in stem and loop regions on the structure and function of adenovirus VA RNAI.

Please wait, while we are loading the content...

Year: 2016, Volume: 35

Year: 2015, Volume: 34

Year: 2014, Volume: 33

Year: 2013, Volume: 33

Year: 2013, Volume: 32

Year: 2012, Volume: 32

Year: 2012, Volume: 31

Year: 2011, Volume: 30

Year: 2010, Volume: 29

Year: 2009, Volume: 28

Year: 2008, Volume: 27

Year: 2007, Volume: 26

Year: 2006, Volume: 26

Year: 2006, Volume: 25

Year: 2005, Volume: 24

Year: 2004, Volume: 24

Year: 2004, Volume: 23

Year: 2003, Volume: 22

Year: 2002, Volume: 21

Year: 2001, Volume: 20

Year: 2000, Volume: 19

Year: 1999, Volume: 18

Year: 1998, Volume: 17

Year: 1997, Volume: 16

Year: 1996, Volume: 15

Year: 1995, Volume: 14

Year: 1994, Volume: 13

Year: 1993, Volume: 12

Year: 1992, Volume: 11

Year: 1991, Volume: 10

Year: 1990, Volume: 9

Year: 1989, Volume: 8

Year: 1988, Volume: 7

Year: 1988, Volume: 7, Issue: 13

Year: 1988, Volume: 7, Issue: 12

Year: 1988, Volume: 7, Issue: 11

Year: 1988, Volume: 7, Issue: 10

Year: 1988, Volume: 7, Issue: 9

Single genes from Agrobacterium rhizogenes influence plant development

In vivo import of plastocyanin and a fusion protein into developmentally different plastids of transgenic plants

Proteins encoded by a complex chloroplast transcription unit are each translated from both monocistronic and polycistronic mRNAs.

Identification of regions affecting virulence, RNA processing and infectivity in the virulent satellite of turnip crinkle virus.

Oscillations in microtubule polymerization: the rate of GTP regeneration on tubulin controls the period.

Attachment, spreading and locomotion of avian neural crest cells are mediated by multiple adhesion sites on fibronectin molecules.

Isolation, immunochemical characterization and localization of the kainate sub-class of glutamate receptor from chick cerebellum.

Isolation and structural analysis of a peptide containing the novel tyrosyl-glucose linkage in glycogenin.

The genes for the alpha 1(IV) and alpha 2(IV) chains of human basement membrane collagen type IV are arranged head-to-head and separated by a bidirectional promoter of unique structure.

The structure of cytochrome b561, a secretory vesicle-specific electron transport protein.

Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme.

Multiple transcripts of the mouse tyrosinase gene are generated by alternative splicing.

Functional analysis of alternatively spliced tyrosinase gene transcripts.

Variants of human tissue-type plasminogen activator that lack specific structural domains of the heavy chain.

Temporal relationship between inositol polyphosphate formation and increases in cytosolic Ca2+ in quiescent 3T3 cells stimulated by platelet-derived growth factor, bombesin and vasopressin.

Expression of functional human EGF receptor on murine bone marrow cells.

Tissue-specific and steroid-dependent interaction of transcription factors with the oestrogen-inducible apoVLDL II promoter in vivo.

Nucleotide sequences of chimpanzee MHC class I alleles: evidence for trans-species mode of evolution.

A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer.

Multiple mechanisms regulate c-myc gene expression during normal T cell activation.

Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.

The promoter of Alzheimer's disease amyloid A4 precursor gene.

The bovine papillomavirus P2443 promoter is E2 trans-responsive: evidence for E2 autoregulation.

Structural and mutational analysis of E2 trans-activating proteins of papillomaviruses reveals three distinct functional domains.

Mutational analysis of the 5' non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation.

Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice.

Effects of mutations in stem and loop regions on the structure and function of adenovirus VA RNAI.

Structure-function relationships in the GTP binding domain of EF-Tu: mutation of Val20, the residue homologous to position 12 in p21.

Scanning independent ribosomal initiation of the Sendai virus X protein.

Seventy-kilodalton heat shock proteins and an additional component from reticulocyte lysate stimulate import of M13 procoat protein into microsomes.

Differential regulation of the two transcripts from the Drosophila gap segmentation gene hunchback.

Neuronal acetylcholine receptors in Drosophila: the ARD protein is a component of a high-affinity alpha-bungarotoxin binding complex.

Temperature effects on molecular processes which lead to stage differentiation in Leishmania.

Collagen-like sequences stabilize homotrimers of a bacterial hydrolase.

Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome.

PL of coliphage lambda: an alternative solution for an efficient promoter.

Isolation of a prokaryotic photoreceptor: sensory rhodopsin from halobacteria

Transposon Tn554 encodes three products required for transposition.

Year: 1988, Volume: 7, Issue: 8

Year: 1988, Volume: 7, Issue: 7

Year: 1988, Volume: 7, Issue: 6

Year: 1988, Volume: 7, Issue: 5

Year: 1988, Volume: 7, Issue: 4

Year: 1988, Volume: 7, Issue: 3

Year: 1988, Volume: 7, Issue: 2

Year: 1988, Volume: 7, Issue: 1

Year: 1987, Volume: 6

Year: 1986, Volume: 5

Year: 1985, Volume: 4

Year: 1984, Volume: 3

Year: 1983, Volume: 2

Year: 1982, Volume: 1

Effects of mutations in stem and loop regions on the structure and function of adenovirus VA RNAI.

Content Provider	PubMed Central
Author	Mellits, K. H. Mathews, M. B.
Abstract	Adenovirus virus-associated (VA) RNAI is required for efficient protein synthesis at late times of adenoviral infection, and in some other situations where double-stranded RNA (dsRNA) is present. It prevents inhibition of protein synthesis by a dsRNA-activated protein kinase and the secondary structure of VA RNAI is though to be important for its activity. To test this idea and to define structures and sequences responsible for VA RNAI activity, we constructed several mutant VA RNA genes and tested them in a transient expression assay. Activity is unaffected by deletions within a small region near the center of the gene, nt 72-85, but it is greatly diminished by deletion or substitution of sequences on the 3' side of this region. The structures of wild-type and mutant RNAs were examined by nuclease-sensitivity analysis. We propose a model for wild-type VA RNAI which differs from that predicted to be the most stable structure. Surprisingly disruption of the longest duplex region in the molecule is tolerated, provided that adjacent structural elements are not rearranged. However, perturbations of elements located in the center of the structure correlate well with loss of function.
Starting Page	2849
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	9
Volume Number	7
Language	English
Publisher Date	1988-09-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in