NDLI: Assessing the bipotency of in vitro-derived neuromesodermal progenitors

Content Provider	PubMed Central
Author	Anestis, Tsakiridis Wilson, Valerie
Copyright Year	2015
Abstract	Retrospective clonal analysis in the mouse has demonstrated that the posterior spinal cord neurectoderm and paraxial mesoderm share a common bipotent progenitor. These neuromesodermal progenitors (NMPs) are the source of new axial structures during embryonic rostrocaudal axis elongation and are marked by the simultaneous co-expression of the transcription factors T(Brachyury) (T(Bra)) and Sox2. NMP-like cells have recently been derived from pluripotent stem cellsin vitro following combined stimulation of Wnt and fibroblast growth factor (FGF) signaling. Under these conditions the majority of cultures consist of T(Bra)/Sox2 co-expressing cells after 48-72 hours of differentiation. Although the capacity of these cells to generate posterior neural and paraxial mesoderm derivatives has been demonstrated at the population level, it is unknown whether a singlein vitro-derived NMP can give rise to both neural and mesodermal cells. Here we demonstrate that T(Bra) positive cells obtained from mouse epiblast stem cells (EpiSCs) after culture in NMP-inducing conditions can generate both neural and mesodermal clones. This finding suggests that, similar to their embryonic counterparts,in vitro-derived NMPs are truly bipotent and can thus be exploited as a model for studying the molecular basis of developmental cell fate decisions.
Related Links	http://dx.doi.org/10.12688/f1000research.6345.1
Starting Page	100
File Format	PDF
ISSN	20461402
e-ISSN	20461402
Journal	F1000Research
Volume Number	4
Language	English
Publisher	F1000Research
Publisher Date	2015-04-01
Access Restriction	Open
Rights Holder	F1000Research
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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