NDLI: A Cyclic GMP-Dependent K+ Channel in the Blastocladiomycete Fungus Blastocladiella emersonii

Content Provider	PubMed Central
Author	Avelar, Gabriela Mól Glaser, Talita Leonard, Guy Richards, Thomas A. Ulrich, Henning Gomes, Suely L.
Copyright Year	2015
Abstract	Phototaxis in flagellated zoospores of the aquatic fungus Blastocladiella emersonii depends on a novel photosensor, Blastocladiella emersonii GC1 (BeGC1), comprising a type I (microbial) rhodopsin fused to a guanylyl cyclase catalytic domain, that produces the conserved second messenger cyclic GMP (cGMP). The rapid and transient increase in cGMP levels during the exposure of zoospores to green light was shown to be necessary for phototaxis and dependent on both rhodopsin function and guanylyl cyclase activity. It is noteworthy that BeGC1 was localized to the zoospore eyespot apparatus, in agreement with its role in the phototactic response. A putative cyclic nucleotide-gated channel (BeCNG1) was also identified in the genome of the fungus and was implicated in flagellar beating via the action of a specific inhibitor (l-cis-diltiazem) that compromised zoospore motility. Here we show that B. emersonii expresses a K+ channel that is activated by cGMP. The use of specific channel inhibitors confirmed the activation of the channel by cGMP and its K+ selectivity. These characteristics are consistent with the function of an ion channel encoded by the BeCNG1 gene. Other blastocladiomycete fungi, such as Allomyces macrogynus and Catenaria anguillulae, possess genes encoding a similar K+ channel and the rhodopsin–guanylyl cyclase fusion protein, while the genes encoding both these proteins are absent in nonflagellated fungi. The presence of these genes as a pair seems to be an exclusive feature of blastocladiomycete fungi. Taken together, these data demonstrate that the B. emersonii cGMP-activated K+ channel is involved in the control of zoospore motility, most probably participating in the cGMP-signaling pathway for the phototactic response of the fungus.
Related Links	http://dx.doi.org/10.1128/EC.00087-15
Ending Page	963
Page Count	6
Starting Page	958
File Format	PDF
ISSN	15359786
e-ISSN	15359786
Journal	Eukaryotic Cell
Issue Number	9
Volume Number	14
Language	English
Publisher	American Society for Microbiology
Publisher Date	2015-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Cyclic AMP binding proteins and cyclic AMP-dependent protein kinase from Blastocladiella emersonii.

Circulation of potassium across the plasma membrane of Blastocladiella emersonii: K+ channel.

Transcriptional Response to Hypoxia in the Aquatic Fungus Blastocladiella emersonii ▿ †

Global Gene Expression Analysis during Sporulation of the Aquatic Fungus Blastocladiella emersonii ▿ †

In Vitro Ribonucleic Acid Synthesis in the Zoospores of the Aquatic Fungus Blastocladiella emersonii

Transcriptome Analysis in Response to Heat Shock and Cadmium in the Aquatic Fungus Blastocladiella emersonii ▿ †

Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii

Characterization and Submitochondrial Localization of the α Subunit of the Mitochondrial Processing Peptidase from the Aquatic Fungus Blastocladiella emersonii

Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii.

A Cyclic GMP-Dependent K+ Channel in the Blastocladiomycete Fungus Blastocladiella emersonii

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