NDLI: A calibration curve for immobilized dihydrofolate reductase activity assay

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A calibration curve for immobilized dihydrofolate reductase activity assay

Content Provider	PubMed Central
Author	Singh, Priyanka Morris, Holly Tivanski, Alexei V. Kohen, Amnon
Copyright Year	2015
Abstract	An assay was developed for measuring the active-site concentration, activity, and thereby the catalytic turnover rate (k cat) of an immobilized dihydrofolate reductase model system (Singh et al., (2015), Anal. Biochem). This data article contains a calibration plot for the developed assay. In the calibration plot rate is plotted as a function of DHFR concentration and shows linear relationship. The concentration of immobilized enzyme was varied by using 5 different size mica chips. The dsDNA concentration was the same for all chips, assuming that the surface area of the mica chip dictates the resulting amount of bound enzyme (i.e. larger sized chip would have more bound DHFR). The activity and concentration of each chip was measured.
Related Links	http://dx.doi.org/10.1016/j.dib.2015.04.005
Ending Page	21
Page Count	3
Starting Page	19
File Format	PDF
ISSN	23523409
e-ISSN	23523409
Journal	Data in Brief
Volume Number	4
Language	English
Publisher	Elsevier
Publisher Date	2015-09-01
Access Restriction	Open
Rights Holder	Elsevier
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Education

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