NDLI: Divergent roles of BECN1 in LC3 lipidation and autophagosomal function

Content Provider	PubMed Central
Author	He, Ruina Peng, Jingyu Yuan, Pengfei Xu, Fang Wei, Wensheng
Copyright Year	2015
Abstract	BECN1/Beclin 1 is regarded as a critical component in the class III phosphatidylinositol 3-kinase (PtdIns3K) complex to trigger autophagy in mammalian cells. Despite its significant role in a number of cellular and physiological processes, the exact function of BECN1 in autophagy remains controversial. Here we created a BECN1 knockout human cell line using the TALEN technique. Surprisingly, the complete loss of BECN1 had little effect on LC3 (MAP1LC3B/LC3B) lipidation, and LC3B puncta resembling autophagosomes by fluorescence microscopy were still evident albeit significantly smaller than those in the wild-type cells. Electron microscopy (EM) analysis revealed that BECN1 deficiency led to malformed autophagosome-like structures containing multiple layers of membranes under amino acid starvation. We further confirmed that the PtdIns3K complex activity and autophagy flux were disrupted in BECN1 −/− cells. Our results demonstrate the essential role of BECN1 in the functional formation of autophagosomes, but not in LC3B lipidation.
Related Links	http://dx.doi.org/10.1080/15548627.2015.1034404
Ending Page	747
Page Count	8
Starting Page	740
File Format	PDF
ISSN	15548627
e-ISSN	15548635
Journal	Autophagy
Issue Number	5
Volume Number	11
Language	English
Publisher	Taylor & Francis
Publisher Date	2015-05-04
Access Restriction	Open
Rights Holder	Taylor & Francis
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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