NDLI: Cooperative roles of biological flow and surface topography in guiding sperm migration revealed by a microfluidic model

Content Provider	PubMed Central
Author	Tung, Chih-kuan Ardon, Florencia Fiore, Alyssa Suarez, Susan S. Wu, Mingming
Copyright Year	2013
Abstract	Successful reproduction in mammals requires sperm to swim against a fluid flow and through the long and complex female reproductive tract before reaching the egg in the oviduct. Millions of them do not make it. Despite the clinical importance, the roles played in sperm migration by the diverse biophysical and biochemical microenvironments within the reproductive tract are largely unknown. In this article, we present the development of a double layer microfluidic device that recreates two important biophysical environments within female reproductive tract: fluid flow and surface topography. The unique feature of the device is that it enables one to study the cooperative roles of fluid flow and surface topography in guiding sperm migration. Using bull sperm as a model system, we found that microfluidic grooves embedded on a channel surface facilitate sperm migration against fluid flows. These findings suggest ways to design in vitro fertilization devices to treat infertility and to develop non-invasive contraceptives that use microarchitectural design to entrap sperm.
Related Links	http://dx.doi.org/10.1039/c3lc51297e
Ending Page	1356
Page Count	9
Starting Page	1348
File Format	PDF
ISSN	14730197
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	7
Volume Number	14
Language	English
Publisher Date	2014-01-01
Access Restriction	Open
Subject Keyword	Biochemistry Bioengineering Chemistry(all) Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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