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Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform
| Content Provider | PubMed Central |
|---|---|
| Author | Chen, Rui Im, Hogune Snyder, Michael |
| Copyright Year | 2015 |
| Abstract | Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Illumina TruSeq Exome Enrichment platform, which captures ~62 Mb of the human exonic regions using 95-base DNA probes. In addition to covering the RefSeq and Ensembl coding sequences, the enriched sequences also include ~28 Mb of RefSeq untranslated regions (UTR). The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer. |
| Related Links | http://dx.doi.org/10.1101/pdb.prot084863 |
| File Format | |
| ISSN | 15596095 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor protocols |
| Issue Number | 7 |
| Volume Number | 2015 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |