NDLI: Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression

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Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression

Content Provider	PubMed Central
Author	Fujita, Katsuhide Fukuda, Makiko Fukui, Hiroko Horie, Masanori Endoh, Shigehisa Uchida, Kunio Shichiri, Mototada Morimoto, Yasuo Ogami, Akira Hitoshi, Iwahashi
Copyright Year	2015
Abstract	The use of carbon nanotubes in the industry has grown; however, little is known about their toxicological mechanism of action. Single-wall carbon nanotube (SWCNT) suspensions were administered by single intratracheal instillation in rats. Persistence of alveolar macrophage-containing granuloma was observed around the sites of SWCNT aggregation at 90 days post-instillation in 0.2-mg- or 0.4-mg-injected doses per rat. Meanwhile, gene expression profiling revealed that a large number of genes involved in the inflammatory response were markedly upregulated until 90 days or 180 days post-instillation. Subsequently, gene expression patterns were dramatically altered at 365 days post-instillation, and the number of upregulated genes involved in the inflammatory response was reduced. These results suggested that alveolar macrophage-containing granuloma reflected a characteristic of the histopathological transition period from the acute-phase to the subchronic-phase of inflammation, as well as pulmonary acute phase response persistence up to 90 or 180 days after intratracheal instillation in this experimental setting. The expression levels of the genes Ctsk, Gcgr, Gpnmb, Lilrb4, Marco, Mreg, Mt3, Padi1, Slc26a4, Spp1, Tnfsf4 and Trem2 were persistently upregulated in a dose-dependent manner until 365 days post-instillation. In addition, the expression levels of Atp6v0d2, Lpo, Mmp7, Mmp12 and Rnase9 were significantly upregulated until 754 days post-instillation. We propose that these persistently upregulated genes in the chronic-phase response following the acute-phase response act as potential biomarkers in lung tissue after SWCNT instillation. This study provides further insight into the time-dependent changes in genomic expression associated with the pulmonary toxicity of SWCNTs.
Related Links	http://dx.doi.org/10.3109/17435390.2014.921737
Ending Page	301
Page Count	12
Starting Page	290
File Format	PDF
ISSN	17435390
e-ISSN	17435404
Journal	Nanotoxicology
Issue Number	3
Volume Number	9
Language	English
Publisher	Informa Healthcare
Publisher Date	2015-04-03
Access Restriction	Open
Rights Holder	Informa Healthcare
Subject Keyword	Toxicology Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Toxicology Biomedical Engineering

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