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| Content Provider | PubMed Central |
|---|---|
| Author | Hepojoki, Satu Rusanen, Juuso Hepojoki, Jussi Nurmi, Visa Antti, Vaheri Lundkvist, Ã ke Hedman, Klaus Olli, Vapalahti |
| Editor | Loeffelholz, M. J. |
| Copyright Year | 2015 |
| Abstract | In this study, we describe a competitive homogeneous immunoassay that makes use of Förster resonance energy transfer (FRET) in rapid detection of pathogen-specific antibodies. The assay principle is based on competition between a monoclonal antibody (MAb) and serum antibodies to a given antigen. In the assay, named competitive FRET immunoassay (CFRET-IA), the FRET signal is induced if MAb carrying a donor label binds to an acceptor-labeled antigen. Specific antibodies in serum compete for antigen binding, resulting in reduced FRET signal. The proof-of-principle for the assay was obtained using donor-labeled Puumala virus nucleocapsid protein (PUUV-N) and acceptor-labeled anti-PUUV-N MAb. The assay was evaluated by analyzing 329 clinical samples comprising 101 from individuals with acute PUUV infection, 42 from individuals with past infection, and 186 from individuals with PUUV-seronegative sera, and the results were compared to those of reference tests. The rapid serodiagnostic test we introduced herein performed with 100% sensitivity and 99% specificity for diagnosing acute hantavirus disease. |
| Related Links | http://dx.doi.org/10.1128/jcm.00663-15 |
| Ending Page | 2297 |
| Page Count | 6 |
| Starting Page | 2292 |
| File Format | |
| ISSN | 00951137 |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 7 |
| Volume Number | 53 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2015-07-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Microbiology (medical) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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