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Evaluating Filamentous Yeast Growth at the Single-Cell Level
| Content Provider | PubMed Central |
|---|---|
| Author | Cullen, Paul J. |
| Copyright Year | 2015 |
| Abstract | Budding yeast can undergo filamentous growth in response to nutrient limitation. Filament formation can be examined under glucose-limiting conditions by the single-cell invasive growth assay, or under nitrogen-limiting conditions with the pseudohyphal growth assay, both described here. The single-cell assay allows robust quantitation of changes in budding pattern and cell length, and most cells in the population show the response. The pseudohyphal growth assay reveals filamentous patterns in larger microcolonies and adjoining subpopulations of cells. Historically, the single-cell assay has been used to study filamentous growth in haploid cells, and the pseudohyphal growth assay in diploid cells. However, both assays can be used in either cell type. |
| Related Links | http://dx.doi.org/10.1101/pdb.prot085084 |
| Ending Page | 275 |
| Page Count | 4 |
| Starting Page | 272 |
| File Format | |
| ISSN | 19403402 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor protocols |
| Issue Number | 3 |
| Volume Number | 2015 |
| Language | English |
| Publisher Date | 2015-03-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry, Genetics and Molecular Biology(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
