NDLI: Mitochondrial membrane potential is regulated by vimentin intermediate filaments

Content Provider	PubMed Central
Author	Chernoivanenko, Ivan S. Matveeva, Elena A. Gelfand, Vladimir I. Goldman, Robert D. Minin, Alexander A.
Copyright Year	2015
Abstract	This study demonstrates that the association of mitochondria with vimentin intermediate filaments (VIFs) measurably increases their membrane potential. This increase is detected by quantitatively comparing the fluorescence intensity of mitochondria stained with the membrane potential-sensitive dye tetramethylrhodamine-ethyl ester (TMRE) in murine vimentin-null fibroblasts with that in the same cells expressing human vimentin (∼35% rise). When vimentin expression is silenced by small hairpin RNA (shRNA) to reduce vimentin by 90%, the fluorescence intensity of mitochondria decreases by 20%. The increase in membrane potential is caused by specific interactions between a subdomain of the non-α-helical N terminus (residues 40 to 93) of vimentin and mitochondria. In rho 0 cells lacking mitochondrial DNA (mtDNA) and consequently missing several key proteins in the mitochondrial respiratory chain (ρ 0 cells), the membrane potential generated by an alternative anaerobic process is insensitive to the interactions between mitochondria and VIF. The results of our studies show that the close association between mitochondria and VIF is important both for determining their position in cells and their physiologic activity.—Chernoivanenko, I. S., Matveeva, E. A., Gelfand, V. I., Goldman, R. D., Minin, A. A. Mitochondrial membrane potential is regulated by vimentin intermediate filaments.
Related Links	http://dx.doi.org/10.1096/fj.14-259903
Ending Page	827
Page Count	8
Starting Page	820
File Format	PDF
ISSN	08926638
e-ISSN	15306860
Journal	The FASEB Journal
Issue Number	3
Volume Number	29
Language	English
Publisher	Federation of American Societies for Experimental Biology
Publisher Date	2015-03-01
Access Restriction	Open
Rights Holder	Federation of American Societies for Experimental Biology
Subject Keyword	Biotechnology Genetics Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Biochemistry Biotechnology

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