NDLI: Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1

Content Provider	PubMed Central
Author	Sugawara, Neal Goldfarb, Tamara Barbara, Studamire Eric, Alani Haber, James E.
Copyright Year	2004
Abstract	Recombination between moderately divergent DNA sequences is impaired compared with identical sequences. In yeast, an HO endonuclease-induced double-strand break can be repaired by single-strand annealing (SSA) between flanking homologous sequences. A 3% sequence divergence between 205-bp sequences flanking the double-strand break caused a 6-fold reduction in repair compared with identical sequences. This reduction in heteroduplex rejection was suppressed in a mismatch repair-defective msh6Δ strain and partially suppressed in an msh2 separation-of-function mutant. In mlh1Δ strains, heteroduplex rejection was greater than in msh6Δ strains but less than in wild type. Deleting PMS1, MLH2,or MLH3 had no effect on heteroduplex rejection, but a pms1Δ mlh2Δ mlh3Δ triple mutant resembled mlh1Δ. However, correction of the mismatches within heteroduplex SSA intermediates required PMS1 and MLH1 to the same extent as MSH2 and MSH6. An SSA competition assay in which either diverged or identical repeats can be used for repair showed that heteroduplex DNA is likely to be unwound rather than degraded. This conclusion is supported by the finding that deleting the SGS1 helicase also suppressed heteroduplex rejection.
Related Links	http://dx.doi.org/10.1073/pnas.0305749101
Starting Page	9315
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	101
Language	English
Publisher	National Academy of Sciences
Publisher Date	2004-06-22
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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