NDLI: Reconfigurable Microfluidics with Integrated Aptasensors for Monitoring Intercellular Communication

Content Provider	PubMed Central
Author	Kwa, Timothy Zhou, Qing Gao, Yandong Rahimian, Ali Kwon, Lydia Liu, Ying Revzin, Alexander
Abstract	We report the development of a microsystem integrating anti-TNF-α aptasensors with vacuum-actuatable microfluidic devices that may be used to monitor intercellular communications. Actuatable chambers were used to expose to mitogen a group of ∼600 cells while not stimulating another group of monocytes only 600μm away. Co-localizing groups of cells with miniature 300μm diameter aptamer-modified electrodes enabled monitoring TNF-α release from each group independently. The microsystem allowed to observe the sequence of events that included 1) mitogenic activation of the first group of monocytes to produce TNF-α, 2) diffusion of TNF-α to the location of the second group of cells and 3) activation of the second group of cells resulting in production of TNF-α by these cells. Thus we were able to experimentally verify reciprocal paracrine cross-talk between the two groups of cells secretion the same signalling molecule. Given the prevalence of such cellular communications during injury, cancer or immune response and the dearth of available monitoring techniques, the microsystem described here is envisioned to have significant impact on cell biology.
Related Links	http://dx.doi.org/10.1039/c4lc00037d
Ending Page	1704
Page Count	10
Starting Page	1695
File Format	PDF
ISSN	14730189
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	10
Volume Number	14
Language	English
Publisher Date	2014-05-21
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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