NDLI: A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella

Content Provider	PubMed Central
Author	Wei, Li Tang, Waixing Teves, Maria E. Zhang, Zhengang Zhang, Ling Li, Hongfei Archer, Kellie J. Peterson, Darrell L. Williams, David C. Strauss, Jerome F. Zhang, Zhibing
Copyright Year	2015
Abstract	A key event in the process of spermiogenesis is the formation of the flagella, which enables sperm to reach eggs for fertilization. Yeast two-hybrid studies revealed that meiosis-expressed gene 1 (MEIG1) and Parkin co-regulated gene (PACRG) interact, and that sperm-associated antigen 16, which encodes an axoneme central apparatus protein, is also a binding partner of MEIG1. In spermatocytes of wild-type mice, MEIG1 is expressed in the whole germ cell bodies, but the protein migrates to the manchette, a unique structure at the base of elongating spermatid that directs formation of the flagella. In the elongating spermatids of wild-type mice, PACRG colocalizes with α-tubulin, a marker for the manchette, whereas this localization was not changed in the few remaining elongating spermatids of Meig1-deficient mice. In addition, MEIG1 no longer localizes to the manchette in the remaining elongating spermatids of Pacrg-deficient mice, indicating that PACRG recruits MEIG1 to the manchette. PACRG is not stable in mammalian cells, but can be stabilized by MEIG1 or by inhibition of proteasome function. SPAG16L is present in the spermatocyte cytoplasm of wild-type mice, and in the manchette of elongating spermatids, but in the Meig1 or Pacrg-deficient mice, SPAG16L no longer localizes to the manchette. By contrast, MEIG1 and PACRG are still present in the manchette of Spag16L-deficient mice, indicating that SPAG16L is a downstream partner of these two proteins. Together, our studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.
Related Links	http://dx.doi.org/10.1242/dev.119834
Ending Page	930
Page Count	10
Starting Page	921
File Format	PDF
ISSN	14779129
e-ISSN	14779129
Journal	Development (Cambridge, England)
Issue Number	5
Volume Number	142
Language	English
Publisher	The Company of Biologists
Publisher Date	2015-03-01
Access Restriction	Open
Rights Holder	The Company of Biologists
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

RIM-BP3 is a manchette-associated protein essential for spermiogenesis

Crystal structure of human PACRG in complex with MEIG1

Dissecting the structural basis of MEIG1 interaction with PACRG

MEIG1 is essential for spermiogenesis in mice

Dissecting the structural basis of MEIG1 interaction with PACRG

The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis

Calaxin is essential for the transmission of Ca2+-dependent asymmetric waves in sperm flagella

Loss of ACTL7A causes small head sperm by defective acrosome-acroplaxome-manchette complex

Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration

A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella

Similar Documents

RIM-BP3 is a manchette-associated protein essential for spermiogenesis

Crystal structure of human PACRG in complex with MEIG1

Dissecting the structural basis of MEIG1 interaction with PACRG

MEIG1 is essential for spermiogenesis in mice

Dissecting the structural basis of MEIG1 interaction with PACRG

The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis

Calaxin is essential for the transmission of Ca2+-dependent asymmetric waves in sperm flagella

Loss of ACTL7A causes small head sperm by defective acrosome-acroplaxome-manchette complex

Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration

A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella