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| Content Provider | PubMed Central |
|---|---|
| Author | Yuet, Kai P. Doma, Meenakshi K. Ngo, John T. Sweredoski, Michael J. Graham, Robert L. J. Moradian, Annie Hess, Sonja Schuman, Erin M. Sternberg, Paul W. Tirrell, David A. |
| Abstract | The emergence of mass spectrometry-based proteomics has revolutionized the study of proteins and their abundances, functions, interactions, and modifications. However, it is difficult to monitor dynamic changes in protein synthesis in a specific cell type within its native environment. Here we describe a method that enables the metabolic labeling, purification, and analysis of proteins in specific cell types and during defined periods in live animals. Using Caenorhabditis elegans, we show that labeling can be restricted to body wall muscles, intestinal epithelial cells, neurons, pharyngeal muscle, and cells that respond to heat shock. By coupling our methodology with isotopic labeling, we successfully identify proteins—including proteins with previously unknown expression patterns—expressed in targeted subsets of cells. |
| Related Links | http://dx.doi.org/10.1073/pnas.1421567112 |
| Ending Page | 2710 |
| Page Count | 6 |
| Starting Page | 2705 |
| File Format | |
| ISSN | 10916490 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 9 |
| Volume Number | 112 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2015-03-03 |
| Access Restriction | Open |
| Rights Holder | National Academy of Sciences |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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