NDLI: Nuclear-mitotic apparatus protein: a structuralprotein interface between the nucleoskeleton and RNA splicing.

Content Provider	PubMed Central
Author	Zeng, C. He, D. Berget, S. M. Brinkley, B. R.
Abstract	Vertebrate splicing factors are localized todiscrete domains within the nuclei of somatic cells. The mechanism whereby suchnuclear domains, identified as speckles by immunofluorescence microscopy, aregenerated is unclear. Recent studies suggest that the spatial order within thenucleus is maintained by nuclear matrix factors. Here we show that a protein inthe nuclear matrix and mitotic apparatus [nuclear-mitotic apparatus protein,NuMA; Lydersen, B. & Pettijohn, D. (1980) Cell 22, 489-499] colocalizes withsplicing factors in interphase nuclei and is associated with small nuclearribonucleoproteins in a complex immunoprecipitated from HeLa extract with smallnuclear ribonucleoprotein antibodies. Moreover, NuMA associates with splicingcomplexes that are reconstituted in vitro using wild-type pre-mRNA, but not withnonspecific RNA. Cumulatively, these observations suggest a function of NuMA orNuMA-like proteins in interphase cells in providing a bridge between RNAprocessing and the nucleoskeleton.
Starting Page	1505
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	91
Language	English
Publisher Date	1994-02-15
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Nuclear-mitotic apparatus protein: a structuralprotein interface between the nucleoskeleton and RNA splicing.