NDLI: Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

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Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

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Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

Content Provider	PubMed Central
Author	Tomasz, Rewicz Wattier, Rémi A. Rigaud, Thierry Bacela-spychalska, Karolina Grabowski, Michal
Abstract	Dikerogammarus villosus is a freshwater amphipod of the Ponto-Caspian origin recognized as one of the 100 worst alien species in Europe, having negative impact on biodiversity and functioning of the invaded aquatic ecosystems. The species has a wide ecophysiological tolerance and during the last 20 years it has rapidly spread throughout European inland waters. In consequence, it presents a major conservation management problem. We describe eight polymorphic microsatellite loci developed for D. villosus by combining a biotin-enrichment protocol and new generation 454GS-FLX Titanium pyrosequencing technology. When genotyped in 64 individuals from two locations, the loci exhibited a mean diversity of 4.87 alleles per locus (2–13). The mean observed and expected heterozygosities were, respectively, 0.439 (0.091–0.844) and 0.468 (0.089–0.843). Gametic disequilibrium was not detected for any pair of loci. The microsatellite markers will be a valuable tool in assessing the demographic processes associated with invasion of the killer shrimp from a genetic point of view.
Related Links	http://dx.doi.org/10.1007/s11033-014-3742-0
Ending Page	17
Page Count	5
Starting Page	13
File Format	PDF
ISSN	03014851
e-ISSN	15734978
Journal	Molecular Biology Reports
Volume Number	42
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-01-01
Access Restriction	Open
Rights Holder	Springer Netherlands
Subject Keyword	Genetics Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

Pilferer, murderer of innocents or prey? The potential impact of killer shrimp (Dikerogammarus villosus) on crayfish

First records of two formerly overlooked Ponto-Caspian amphipods from Turkey: Echinogammarus trichiatus (Martynov, 1932) and Dikerogammarus villosus (Sovinsky, 1894)

First records of two formerly overlooked Ponto-Caspian amphipods from Turkey: Echinogammarus trichiatus (Martynov, 1932) and Dikerogammarus villosus (Sovinsky, 1894)

Feeding on micro-algae in the invasive Ponto-Caspian amphipod Dikerogammarus villosus (Sowinsky 1984)

River-specific effects of the invasive amphipod Dikerogammarus villosus (Crustacea: Amphipoda) on benthic communities

Feeding on micro-algae in the invasive Ponto-Caspian amphipod Dikerogammarus villosus (Sowinsky 1984)

River-specific effects of the invasive amphipod Dikerogammarus villosus (Crustacea: Amphipoda) on benthic communities

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

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Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)

Pilferer, murderer of innocents or prey? The potential impact of killer shrimp (Dikerogammarus villosus) on crayfish

First records of two formerly overlooked Ponto-Caspian amphipods from Turkey: Echinogammarus trichiatus (Martynov, 1932) and Dikerogammarus villosus (Sovinsky, 1894)

First records of two formerly overlooked Ponto-Caspian amphipods from Turkey: Echinogammarus trichiatus (Martynov, 1932) and Dikerogammarus villosus (Sovinsky, 1894)

Feeding on micro-algae in the invasive Ponto-Caspian amphipod Dikerogammarus villosus (Sowinsky 1984)

River-specific effects of the invasive amphipod Dikerogammarus villosus (Crustacea: Amphipoda) on benthic communities

Feeding on micro-algae in the invasive Ponto-Caspian amphipod Dikerogammarus villosus (Sowinsky 1984)

River-specific effects of the invasive amphipod Dikerogammarus villosus (Crustacea: Amphipoda) on benthic communities

Isolation and characterization of 8 microsatellite loci for the “killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)