NDLI: Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: The power of native nanodiscs

Content Provider	PubMed Central
Author	Dörr, Jonas M. Koorengevel, Martijn C. Schäfer, Marre Prokofyev, Alexander V. Stefan, Scheidelaar Van, Der Cruijsen Elwin A. W. Dafforn, Timothy R. Baldus, Marc Killian, J. Antoinette
Abstract	The study of membrane proteins is often hampered by their tendency to misfold when extracted by detergent. Here, we explore a detergent-free approach to isolating membrane proteins while retaining their native lipid environment, making use of an amphipathic polymer that solubilizes intact membrane patches in the form of nanodiscs. Using a potassium channel as a model protein, we show that these “native nanodiscs” are highly thermostable particles that are suitable for spectroscopic studies, allowing structural characterization of the protein in its native environment and direct analysis of the lipids in its immediate surroundings. We also demonstrate that the channel can be reconstituted from nanodiscs into planar lipid bilayers for functional characterization, thus making native nanodiscs an excellent alternative to detergent solubilization.
Related Links	http://dx.doi.org/10.1073/pnas.1416205112
Ending Page	18612
Page Count	6
Starting Page	18607
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	52
Volume Number	111
Language	English
Publisher	National Academy of Sciences
Publisher Date	2014-12-30
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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