NDLI: The Role of Histamine in the Retina: Studies on the Hdc Knockout Mouse

Content Provider	PubMed Central
Author	Ursula, Greferath Vessey, Kirstan A. Jobling, Andrew I. Mills, Samuel A. Bui, Bang V. He, Zheng Nag, Nupur Hiroshi, Ohtsu Fletcher, Erica L.
Editor	Kihara, Alexandre Hiroaki
Copyright Year	2014
Abstract	The role of histamine in the retina is not well understood, despite it regulating a number of functions within the brain, including sleep, feeding, energy balance, and anxiety. In this study we characterized the structure and function of the retina in mice that lacked expression of the rate limiting enzyme in the formation of histamine, histidine decarboxylase (Hdc−/− mouse). Using laser capture microdissection, Hdc mRNA expression was assessed in the inner and outer nuclear layers of adult C57Bl6J wildtype (WT) and Hdc−/−-retinae. In adult WT and Hdc−/−-mice, retinal fundi were imaged, retinal structure was assessed using immunocytochemistry and function was probed by electroretinography. Blood flow velocity was assessed by quantifying temporal changes in the dynamic fluorescein angiography in arterioles and venules. In WT retinae, Hdc gene expression was detected in the outer nuclear layer, but not the inner nuclear layer, while the lack of Hdc expression was confirmed in the Hdc−/− retina. Preliminary examination of the fundus and retinal structure of the widely used Hdc−/−mouse strain revealed discrete lesions across the retina that corresponded to areas of photoreceptor abnormality reminiscent of the rd8 (Crb1) mutation. This was confirmed after genotyping and the strain designated Hdcrd8/rd8. In order to determine the effect of the lack of Hdc-alone on the retina, Hdc−/− mice free of the Crb1 mutation were bred. Retinal fundi appeared normal in these animals and there was no difference in retinal structure, macrogliosis, nor any change in microglial characteristics in Hdc−/− compared to wildtype retinae. In addition, retinal function and retinal blood flow dynamics showed no alterations in the Hdc−/− retina. Overall, these results suggest that histamine plays little role in modulating retinal structure and function.
Related Links	http://dx.doi.org/10.1371/journal.pone.0116025
Starting Page	116025
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	12
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-12-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Impact of Temporal Variation on Design and Analysis of Mouse Knockout Phenotyping Studies

TRPM3 Expression in Mouse Retina

On the Role of Histamine Receptors in the Regulation of Circadian Rhythms

Proteomic Interactions in the Mouse Vitreous-Retina Complex

Dynamics of Regulatory Networks in the Developing Mouse Retina

Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina

CX3CL1 (Fractalkine) Protein Expression in Normal and Degenerating Mouse Retina: In Vivo Studies

Spatial Distribution of Excitatory Synapses on the Dendrites of Ganglion Cells in the Mouse Retina

Altered Reward Circuitry in the Norepinephrine Transporter Knockout Mouse

The Role of Histamine in the Retina: Studies on the Hdc Knockout Mouse

Similar Documents

Impact of Temporal Variation on Design and Analysis of Mouse Knockout Phenotyping Studies

TRPM3 Expression in Mouse Retina

On the Role of Histamine Receptors in the Regulation of Circadian Rhythms

Proteomic Interactions in the Mouse Vitreous-Retina Complex

Dynamics of Regulatory Networks in the Developing Mouse Retina

Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina

CX3CL1 (Fractalkine) Protein Expression in Normal and Degenerating Mouse Retina: In Vivo Studies

Spatial Distribution of Excitatory Synapses on the Dendrites of Ganglion Cells in the Mouse Retina

Altered Reward Circuitry in the Norepinephrine Transporter Knockout Mouse

The Role of Histamine in the Retina: Studies on the Hdc Knockout Mouse