NDLI: Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells

Content Provider	PubMed Central
Author	Shelton, Michael Metz, Jeff Liu, Jun Carpenedo, Richard L. Demers, Simon-pierre Stanford, William L. Skerjanc, Ilona S.
Copyright Year	2014
Abstract	Cell therapies treating pathological muscle atrophy or damage requires an adequate quantity of muscle progenitor cells (MPCs) not currently attainable from adult donors. Here, we generate cultures of approximately 90% skeletal myogenic cells by treating human embryonic stem cells (ESCs) with the GSK3 inhibitor CHIR99021 followed by FGF2 and N2 supplements. Gene expression analysis identified progressive expression of mesoderm, somite, dermomyotome, and myotome markers, following patterns of embryonic myogenesis. CHIR99021 enhanced transcript levels of the pan-mesoderm gene T and paraxial-mesoderm genes MSGN1 and TBX6; immunofluorescence confirmed that 91% ± 6% of cells expressed T immediately following treatment. By 7 weeks, 47% ± 3% of cells were MYH+ve myocytes/myotubes surrounded by a 43% ± 4% population of PAX7+ve MPCs, indicating 90% of cells had achieved myogenic identity without any cell sorting. Treatment of mouse ESCs with these factors resulted in similar enhancements of myogenesis. These studies establish a foundation for serum-free and chemically defined monolayer skeletal myogenesis of ESCs.
Related Links	http://dx.doi.org/10.1016/j.stemcr.2014.07.001
Ending Page	529
Page Count	14
Starting Page	516
File Format	PDF
ISSN	22136711
e-ISSN	22136711
Journal	Stem Cell Reports
Issue Number	3
Volume Number	3
Language	English
Publisher	Elsevier
Publisher Date	2014-09-01
Access Restriction	Open
Rights Holder	Elsevier
Subject Keyword	Healthcare Stem Cell Stem Cells International genetics Medicine Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Developmental Biology Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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