NDLI: Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Content Provider	PubMed Central
Author	Yu, Xiaohui Yang, Lifang Cairns, Murray J. Dass, Crispin Edward, Saravolac Xiong, Li Sun, Lun-quan
Copyright Year	2014
Abstract	DNAzymes are a novel class of gene suppressors that selectively bind to an RNA substrate by Watson-Crick base pairing and cleave phosphodiester bonds. To explore the potential for therapeutic use of catalytic DNA molecules, active DNAzymes targeting the bcl-xL gene were generated through a multiplex in vitro selection. The DNAzyme-mediated down-regulation of the bcl-xL expression was demonstrated in various cancer cell lines by Western blots. Treatment of the cells with the active DNAzyme led to increases in percentage of apoptotic cells and cytochrome c release from mitochondria, a hall marker of apoptosis. When combined with chemotherapeutics such as Taxol, the DNAzyme significantly sensitised a panel of cancer cells to apoptosis as measured by cell survival assay. In Taxol-resistant cells, down-regulation of bcl-xL expression by the DNAzyme reversed the chemo-resistant phenotype of the cancer cells. In a xenograft mouse model, the DNAzyme was delivered into the tumors via an ALZET osmotic pump and shown to chemosensitize PC3 tumor when treating with Taxol. The results from the present study demonstrate that bcl-xL DNAzyme treatment facilitates apoptosis in solid tumors and suggest the potential use of bcl-xL DNAzyme in combination with chemotherapeutics for cancer therapy.
Related Links	http://dx.doi.org/10.18632/oncotarget.1996
Ending Page	9048
Page Count	10
Starting Page	9039
File Format	PDF
ISSN	19492553
e-ISSN	19492553
Journal	Oncotarget
Issue Number	19
Volume Number	5
Language	English
Publisher	Impact Journals LLC
Publisher Date	2014-10-01
Access Restriction	Open
Rights Holder	Impact Journals LLC
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Chemosensitization of solid tumors by inhibition of Bcl-xL expression using DNAzyme.

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

The downregulation of Mcl-1 via USP9X inhibition sensitizes solid tumors to Bcl-xl inhibition

The downregulation of Mcl-1 via USP9X inhibition sensitizes solid tumors to Bcl-xl inhibition

Inhibition of Antiapoptotic BCL-XL, BCL-2, and MCL-1 Proteins by Small Molecule Mimetics

Expression of Inducible Bcl-XS in Myeloid Leukemia: Compensatory Upregulation of Bcl-XL and Bcl-2 Prevents Apoptosis and Chemosensitization

Prognostic significance of Bcl-xL expression and efficacy of Bcl-xL targeting therapy in urothelial carcinoma

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Similar Documents

Chemosensitization of solid tumors by inhibition of Bcl-xL expression using DNAzyme.

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme

The downregulation of Mcl-1 via USP9X inhibition sensitizes solid tumors to Bcl-xl inhibition

The downregulation of Mcl-1 via USP9X inhibition sensitizes solid tumors to Bcl-xl inhibition

Inhibition of Antiapoptotic BCL-XL, BCL-2, and MCL-1 Proteins by Small Molecule Mimetics

Expression of Inducible Bcl-XS in Myeloid Leukemia: Compensatory Upregulation of Bcl-XL and Bcl-2 Prevents Apoptosis and Chemosensitization

Prognostic significance of Bcl-xL expression and efficacy of Bcl-xL targeting therapy in urothelial carcinoma

Chemosensitization of Solid Tumors by Inhibition of Bcl-xL Expression Using DNAzyme